Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, Birmingham, AL 35294, USA.
In Vitro Cell Dev Biol Anim. 2010 Feb;46(2):87-91. doi: 10.1007/s11626-009-9248-8.
Osteopontin (OPN) is a secreted glycoprotein implicated to function in cancer development and metastasis. Although elevated expression of OPN are observed in cancer cells of various types, in some cases, only the cells in the stromal region surrounding the tumor express OPN, suggesting distinct functional roles for this protein derived from host cells and from cancer cells. To provide a model for addressing the functions and mechanisms of host-derived OPN in cancer progression and metastasis, a cutaneous squamous cell carcinoma cell line (ONSC) that lacks the OPN gene, Spp1, was established. This line of cells was derived from a squamous cell carcinoma that developed in a female, OPN-null mouse subjected to two-stage skin carcinogenesis. Morphologically, ONSC cells resemble epithelial cells, and they express the epithelial markers, K1, K14, and p63, as confirmed by immunohistochemical analyses. Genomic analyses indicate the presence of mutated H-Ras and p53 genes. ONSC cells form colonies in soft agar and, subcutaneously injected into athymic nude mice, develop into squamous cell carcinomas that metastasize to the lungs. Lacking OPN expression, these squamous cell carcinoma cells provide a model to address the function of host OPN in the context of cancer progression and metastasis.
骨桥蛋白（OPN）是一种分泌型糖蛋白，被认为在癌症的发生和转移中发挥作用。虽然在各种类型的癌细胞中观察到 OPN 的表达升高，但在某些情况下，只有肿瘤周围基质区域的细胞表达 OPN，这表明该蛋白在宿主细胞和癌细胞中具有不同的功能作用。为了提供一个模型来解决宿主来源的 OPN 在癌症进展和转移中的功能和机制，建立了一种缺乏 OPN 基因 Spp1 的皮肤鳞状细胞癌细胞系（ONSC）。这条细胞系来自于一只雌性 OPN 缺失小鼠的皮肤鳞状细胞癌，该小鼠接受了两阶段皮肤致癌作用。形态学上，ONSC 细胞类似于上皮细胞，并且通过免疫组织化学分析证实它们表达上皮标志物 K1、K14 和 p63。基因组分析表明存在突变的 H-Ras 和 p53 基因。ONSC 细胞在软琼脂中形成集落，并在无胸腺裸鼠中皮下注射，发展为鳞状细胞癌，并转移到肺部。由于缺乏 OPN 表达，这些鳞状细胞癌细胞为研究宿主 OPN 在癌症进展和转移中的功能提供了一个模型。
