首次在土耳其检测到的一株人C组轮状病毒的分子特征
Molecular characterization of a human group C rotavirus detected first in Turkey.
作者信息
Mitui Marcelo Takahiro, Bozdayi Gulendam, Dalgic Buket, Bostanci Ilknur, Nishizono Akira, Ahmed Kamruddin
机构信息
Department of Microbiology, School of Medicine, Oita University, Yufu, Oita, Japan.
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
出版信息
Virus Genes. 2009 Oct;39(2):157. doi: 10.1007/s11262-009-0420-8. Epub 2009 Nov 15.
The present study was done to find out the prevalence of group B and C rotavirus infections in children with diarrhea presented at two major hospitals in Ankara, Turkey. Group B rotavirus was not found in any samples. One of 122 samples was positive for group C rotavirus. Phylogenetic analysis of genes for nonstructural protein NSP4, and structural proteins VP4, VP6, and VP7 confirmed the human origin of this strain. Similar to other human group C rotaviruses, one N-glycosylation site was predicted at amino acid residue 67 on the VP7 of strain GUP188. The genes of strain GUP188 were closely related to those of human group C rotavirus strain from the UK (Bristol) for NSP4, China (208 and Wu82) for VP4 and VP6, and from Colombia (Javeriana) for VP7, indicating that the Turkish group C rotavirus was unique and can serve as an additional reference strain for the molecular epidemiology of group C rotaviruses.
本研究旨在查明在土耳其安卡拉两家主要医院就诊的腹泻儿童中B组和C组轮状病毒感染的流行情况。在任何样本中均未发现B组轮状病毒。122份样本中有1份C组轮状病毒呈阳性。对非结构蛋白NSP4以及结构蛋白VP4、VP6和VP7的基因进行系统发育分析，证实了该毒株的人类来源。与其他人类C组轮状病毒相似，在GUP188毒株VP7的氨基酸残基67处预测有一个N-糖基化位点。GUP188毒株的基因与来自英国（布里斯托尔）的NSP4、中国（208和Wu82）的VP4和VP6以及哥伦比亚（哈维里亚纳）的VP7的人类C组轮状病毒毒株的基因密切相关，表明土耳其C组轮状病毒具有独特性，可作为C组轮状病毒分子流行病学的另一个参考毒株。
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