CIMO/Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5301-855 Bragança, Portugal.
SAR QSAR Environ Res. 2009 Jul;20(5-6):579-90. doi: 10.1080/10629360903408423.
Wild mushrooms have been described as sources of natural antioxidants, particularly phenolic compounds. However, many other compounds present in wild mushrooms can also act as antioxidants (reducers), so whole extracts from a wide range of species need to be examined. To gain further knowledge in this area, the relationship between the antioxidant potential (scavenging effect and reducing power) and chemical composition of twenty three samples from seventeen Portuguese wild mushroom species was investigated. A wide range of analytical parameters reported by our research group (including ash, carbohydrates, proteins, fat, monounsaturated fatty acids, polyunsaturated fatty acids, saturated fatty acids, phenolics, flavonoids, ascorbic acid and beta-carotene) were studied and the data were analysed by partial least squares (PLS) regression analysis to allow correlation of all the parameters. Antioxidant activity correlated well with phenolic and flavonoid contents. A QCAR (Quantitative Composition-Activity Relationships) model was constructed, using the PLS method, and its robustness and predictability were verified by internal and external cross-validation methods. Finally, this model proved to be a useful tool in the prediction of mushrooms' reducing power.
野生蘑菇被描述为天然抗氧化剂的来源，特别是酚类化合物。然而，野生蘑菇中存在的许多其他化合物也可以作为抗氧化剂（还原剂），因此需要检查来自广泛物种的全提取物。为了在这一领域获得更多的知识，研究了来自葡萄牙 17 种野生蘑菇的 23 个样本的抗氧化潜力（清除作用和还原能力）与化学成分之间的关系。研究小组报告了广泛的分析参数（包括灰分、碳水化合物、蛋白质、脂肪、单不饱和脂肪酸、多不饱和脂肪酸、饱和脂肪酸、酚类、类黄酮、抗坏血酸和β-胡萝卜素），并通过偏最小二乘（PLS）回归分析对数据进行了分析，以允许所有参数之间的相关性。抗氧化活性与酚类和类黄酮含量密切相关。使用 PLS 方法构建了 QCAR（定量组成-活性关系）模型，并通过内部和外部交叉验证方法验证了其稳健性和预测性。最后，该模型被证明是预测蘑菇还原能力的有用工具。
