Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia.
Biochemistry (Mosc). 2009 Nov;74(11):1225-7. doi: 10.1134/s0006297909110078.
In this work, the diameters of protein complexes formed upon interaction of ceruloplasmin (CP) with lactoferrin (LF) and myeloperoxidase (MPO) were determined. Gage dependence of the diameter of protein particles (myoglobin, albumin, LF, CP, MPO, aldolase, ferritin) on their molecular mass logarithm was calculated. The diameter of a complex formed upon mixing CP and LF was 8.4 nm, which is in line with the radius of gyration obtained previously when the 1CP-1LF complex was studied by small-angle X-ray scattering. The diameter of a complex formed upon interaction of CP with MPO is 9.8 nm, corresponding to the stoichiometry 2CP : 1MPO. The diameter of a complex formed when LF is added to the 2CP-1MPO complex is 10.7 nm. The latter is consistent with the notion of a pentameric structure 2LF-2CP-1MPO with molecular mass of about 585 kDa.
在这项工作中，测定了铜蓝蛋白 (CP) 与乳铁蛋白 (LF) 和髓过氧化物酶 (MPO) 相互作用形成的蛋白质复合物的直径。计算了蛋白质颗粒（肌红蛋白、白蛋白、LF、CP、MPO、醛缩酶、铁蛋白）直径与其分子质量对数的关系。CP 和 LF 混合形成的复合物的直径为 8.4nm，与以前通过小角度 X 射线散射研究 1CP-1LF 复合物时获得的回转半径一致。CP 与 MPO 相互作用形成的复合物的直径为 9.8nm，对应于 2CP:1MPO 的化学计量比。当 LF 加入到 2CP-1MPO 复合物中时，形成的复合物的直径为 10.7nm。后者与约 585kDa 的五聚体结构 2LF-2CP-1MPO 的概念一致。
