Institute of Oral Biology, National Yang Ming University, Taipei, Taiwan.
J Biomed Sci. 2009 Nov 16;16(1):100. doi: 10.1186/1423-0127-16-100.
The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransplantation. SASVO3 possesses enhanced tumorigenic ability both in vitro and in vivo. Moreover, SASVO3 exhibits properties of cancer stem cells, including that increased the abilities of sphere-forming, the number of side population cells, the potential of transplanted tumor growth and elevated expression of the stem cell marker Bmi1. Injection of SASVO3 into the tail vein of nude mice resulted in lung metastases. These results are consistent with the postulate that the malignant and/or metastasis potential of HNSCC cells may reside in a stem-like subpopulation.
实体瘤（包括头颈部鳞状细胞癌）的进展和转移与一小部分肿瘤干细胞的行为有关。在这里，我们通过三轮连续的异种移植，从原发性肿瘤中建立了一个高度恶性的头颈部鳞状细胞癌细胞系 SASVO3。SASVO3 在体外和体内均具有增强的致瘤能力。此外，SASVO3 表现出癌症干细胞的特性，包括增加球体形成能力、侧群细胞数量、移植瘤生长潜能和干细胞标记物 Bmi1 的表达水平升高。将 SASVO3 注入裸鼠尾静脉后，导致肺部转移。这些结果与这样的假设一致，即 HNSCC 细胞的恶性和/或转移潜能可能存在于类似干细胞的亚群中。
