Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology & Institue of Stomatological Research, Sun Yat-sen University, Guangzhou, Guangdong, China.
Cell Biochem Funct. 2009 Dec;27(8):535-41. doi: 10.1002/cbf.1612.
Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population of ESCs, whether p63 can function as a specific marker for ESCs is unclear. We isolated and cultured oral ESCs and illustrated the expression of p63 and its isoforms in rat oral mucosa tissues and stem cells before and after differentiation. Semi-reverse transcription PCR detected the TA, DeltaN, alpha and beta isoforms when cells were cultured for 2 days, but only TA and gamma were detected after 14 days. We also found that p63 is expressed in basal and suprabasal epithelial layers of rat oral mucosa, but it was implied p63 does not mark stem cells specifically, while the DeltaNp63alpha and DeltaNp63beta isoforms may be specific markers of rat oral mucosa stem cells.
虽然已经建立了纯化表皮干细胞（ESCs）的技术，但富集纯的活 ESC 群体仍然是一项具有挑战性的任务。一种提高 ESC 纯度和活力的方法涉及细胞标记物。虽然有证据表明 p63 在维持 ESC 群体中发挥作用，但 p63 是否可以作为 ESC 的特定标记物尚不清楚。我们分离和培养了口腔 ESC，并在分化前后展示了 p63 及其同工型在大鼠口腔黏膜组织和干细胞中的表达。半反转录 PCR 在细胞培养 2 天时检测到 TA、DeltaN、alpha 和 beta 同工型，但在 14 天时仅检测到 TA 和 gamma。我们还发现 p63 在大鼠口腔黏膜的基底和超基底上皮层中表达，但暗示 p63 不是特定的干细胞标记物，而 DeltaNp63alpha 和 DeltaNp63beta 同工型可能是大鼠口腔黏膜干细胞的特异性标记物。
