感染艾滋病毒且患有细菌性阴道病的女性中苛养阴道细菌的检测
Detection of fastidious vaginal bacteria in women with HIV infection and bacterial vaginosis.
作者信息
Mitchell Caroline, Moreira Carla, Fredricks David, Paul Kathleen, Caliendo Angela M, Kurpewski Jaclynn, Ingersoll Jessica, Cu-Uvin Susan
机构信息
Department of Obstetrics & Gynecology, University of Washington, P.O. Box 356460, Seattle, WA 98195, USA.
出版信息
Infect Dis Obstet Gynecol. 2009;2009:236919. doi: 10.1155/2009/236919. Epub 2009 Nov 12.
Abstract
BACKGROUND
Fastidious bacteria have been associated with bacterial vaginosis (BV) using PCR methods. We assessed the prevalence of these bacteria in HIV-1 infected women and their relationship with vaginal pH and shedding of HIV-1 RNA.
METHODS
64 cervicovaginal lavage (CVL) samples were collected from 51 women. Vaginal microbiota were characterized using 8 bacterium-specific quantitative PCR assays.
RESULTS
Women with the fastidious bacteria Bacterial Vaginosis Associated Bacterium (BVAB) 1, 2, and 3 showed a trend to increased HIV-1 shedding (OR 2.59-3.07, P = .14-.17). Absence of Lactobacillus crispatus (P < .005) and presence of BVAB2 (P < .001) were associated with elevated vaginal pH. BVAB1, 2, and 3 were highly specific indicators of BV in HIV-infected women, with specificities of 89%-93%.
CONCLUSIONS
Fastidious bacteria (BVAB 1, 2, and 3) remain specific indicators of BV in HIV-infected women, and BVAB2 may contribute to the elevated vaginal pH that is a hallmark of this syndrome.
摘要
背景
使用聚合酶链反应（PCR）方法已发现苛求菌与细菌性阴道病（BV）有关。我们评估了这些细菌在HIV-1感染女性中的患病率及其与阴道pH值和HIV-1 RNA脱落的关系。
方法
从51名女性中收集了64份宫颈阴道灌洗（CVL）样本。使用8种细菌特异性定量PCR检测方法对阴道微生物群进行了特征分析。
结果
感染苛求菌阴道加德纳菌相关细菌（BVAB）1、2和3的女性显示出HIV-1脱落增加的趋势（比值比2.59 - 3.07，P = 0.14 - 0.17）。卷曲乳杆菌缺失（P < 0.005）和BVAB2存在（P < 0.001）与阴道pH值升高有关。BVAB1、2和3是HIV感染女性中BV的高度特异性指标，特异性为89% - 93%。
结论
苛求菌（BVAB 1、2和3）仍然是HIV感染女性中BV的特异性指标，并且BVAB2可能导致阴道pH值升高，这是该综合征的一个标志。
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