一维和二维高分辨魔角旋转 NMR 光谱法检测人恶性前列腺组织中的多不饱和 ω-6 脂肪酸。
Detection of polyunsaturated omega-6 fatty acid in human malignant prostate tissue by 1D and 2D high-resolution magic angle spinning NMR spectroscopy.
机构信息
Department of Radiation Sciences, Diagnostic Radiology, Umeå University and University Hospital of Northern Sweden, Umeå, 901 87 Sweden.
出版信息
MAGMA. 2009 Dec;22(6):327-31. doi: 10.1007/s10334-009-0187-x. Epub 2009 Nov 17.
OBJECT
Polyunsaturated omega-6 fatty acids (PUFAs) have been shown to promote prostate cancer. Here, we describe the use of HRMAS NMR spectroscopy to detect omega-6 PUFA species in prostate tissues.
MATERIALS AND METHODS
Samples originating from non-malignant (n = 54) and malignant (n = 27) prostate tissues (from 27 prostatectomized men) were studied by 1D (1)H, 2D (1)H-(1)H and (1)H-(13)C HRMAS NMR spectroscopy followed by histopathological characterization.
RESULTS
HRMAS NMR proved to be a powerful, non-destructive method to identify and characterize PUFAs. The omega-6 PUFA was found in 15% of examined human prostate tumors.
CONCLUSION
It is possible to detect PUFAs in prostate tissues using our NMR-based spectroscopic approach.
目的
多不饱和ω-6 脂肪酸（PUFAs）已被证明可促进前列腺癌的发生。在这里，我们描述了使用 HRMAS NMR 光谱法检测前列腺组织中 ω-6 PUFAs 种类的方法。
材料和方法
使用 1D（1）H、2D（1）H-（1）H 和（1）H-（13）C HRMAS NMR 光谱法对源自非恶性（n = 54）和恶性（n = 27）前列腺组织（来自 27 名前列腺切除术患者）的样本进行了研究，并进行了组织病理学特征分析。
结果
HRMAS NMR 被证明是一种强大的、非破坏性的方法，可用于鉴定和表征 PUFAs。在 15%的检查人类前列腺肿瘤中发现了 ω-6 PUFAs。
结论
使用我们基于 NMR 的光谱方法可以在前列腺组织中检测到 PUFAs。
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