Department of Anatomy, Faculty of Veterinary Medicine, Belgrade, Serbia.
Int J Neurosci. 2009;119(8):1228-38. doi: 10.1080/00207450802333870.
The proliferation of hippocampal dentate gyrus granule cells was investigated using (3)H-thymidine incorporation in control and estrogen-treated rats. Newborn 3-day old female Wistar rats were treated with a single dose of 1 mg of estradiol and 30 microCi (3)H-thymidine, and were sacrificed when 10 days old. The total number of neurons and the number of labeled granule cells in the granular layer and its subdivisions of both suprapyramidal and infrapyramidal limbs were analyzed using a stereological method. In both limbs, the total number of neurons as well as the total number of labeled granule cells in the granular layer were significantly increased in treated rats compared to corresponding controls. The thicknesses of the molecular and the granular layers and their subdivisions of both suprapyramidal and infrapyramidal limbs were analyzed using a stereological method. In treated female rats the molecular layer (ML) in both limbs was significantly decreased, and the granular layer (GL) was significantly increased in suprapyramidal limb. However, in the infrapyramidal limb an increased number of labeled cells in treated animals were significant in all particular zones of the granular layer. In the suprapyramidal limb's granular layer a significant increase in labeled cells was observed in subgranular zone (SGZ). Our results suggest a differential effect of estradiol on thicknesses of the ML and the GL, and dentate gyrus granule cells proliferation through the early rat life.
采用（3）H-胸腺嘧啶核苷掺入法研究了海马齿状回颗粒细胞的增殖情况，在对照组和雌激素处理组的大鼠中进行了研究。新生 3 天大的雌性 Wistar 大鼠接受了 1 毫克雌二醇和 30 微 Ci（3）H-胸腺嘧啶核苷的单次剂量治疗，并在 10 天大时处死。使用体视学方法分析了颗粒层及其上、下锥体支的各个亚区的总神经元数和标记的颗粒细胞数。在上、下锥体支的各个亚区中，与相应的对照组相比，处理组的总神经元数和颗粒层中的标记颗粒细胞总数均显著增加。使用体视学方法分析了上、下锥体支的分子层（ML）和颗粒层（GL）及其各个亚区的厚度。在处理后的雌性大鼠中，上、下锥体支的分子层（ML）均显著减小，而上锥体支的颗粒层（GL）显著增加。然而，在下锥体支的颗粒层中，处理动物的所有特定区域的标记细胞数量均显著增加。在上锥体支的颗粒层的颗粒下区（SGZ）中观察到标记细胞的显著增加。我们的结果表明，雌激素对 ML 和 GL 的厚度以及通过早期大鼠生命的齿状回颗粒细胞增殖具有不同的影响。
