近平滑假丝酵母IFO 0708共轭聚酮还原酶C2（CPR-C2）的表达、纯化、结晶及初步X射线分析
Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708.
作者信息
Yamamura Akihiro, Maruoka Shintaro, Ohtsuka Jun, Miyakawa Takuya, Nagata Koji, Kataoka Michihiko, Kitamura Nahoko, Shimizu Sakayu, Tanokura Masaru
机构信息
Department of Applied Biological Chemistry, University of Tokyo, Yayoi, Bunkyo-ku, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1145-8. doi: 10.1107/S1744309109038238. Epub 2009 Oct 30.
Conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708 is a member of the NADPH-dependent aldo-keto reductase (AKR) superfamily and catalyzes the stereospecific reduction of ketopantoyl lactone to d-pantoyl lactone. A diffraction-quality crystal of recombinant CPR-C2 was obtained by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.7 angstrom resolution on beamline NW12A of the Photon Factory-Advanced Ring (Tsukuba, Japan). The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 55.02, b = 68.30, c = 68.93 angstrom. The Matthews coefficient (V(M) = 1.76 angstrom(3) Da(-1)) indicated that the crystal contained one CPR-C2 molecule per asymmetric unit.
近平滑假丝酵母IFO 0708的共轭聚酮还原酶C2（CPR-C2）是NADPH依赖性醛酮还原酶（AKR）超家族的成员，催化酮泛酰内酯立体定向还原为d-泛酰内酯。使用聚乙二醇3350作为沉淀剂，通过坐滴气相扩散法获得了重组CPR-C2的衍射质量晶体。该晶体在日本筑波光子工厂先进环的NW12A光束线上X射线衍射分辨率达到1.7埃。晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 55.02、b = 68.30、c = 68.93埃。马修斯系数（V(M)=1.76埃(3) Da(-1)）表明每个不对称单元的晶体包含一个CPR-C2分子。
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