Ozbek M, Erdogan M, Karadeniz M, Cetinkalp S, Ozgen A G, Saygili F, Yilmaz C, Tuzun M
Ege University Faculty of Medicine, Department of Endocrinology and Metabolism, Izmir, Turkey.
Exp Clin Endocrinol Diabetes. 2009 Nov;117(10):573-6. doi: 10.1055/s-0029-1234087. Epub 2009 Nov 18.
Defective insulin secretion is required for the development of frank diabetes mellitus. We evaluated the secretory response of pancreatic beta cells after the ingestion of mixed meal plus oral L-arginine in newly diagnosed type 2 diabetic patients.
Twenty-four newly diagnosed type 2 diabetic patients were enrolled in this study. All patients were ingested a mixed meal of 553 kcal. Serum insulin levels were measured at time 0 just before the mixed meal and at 1, 2, 3, 4 and 5 h after the ingestion of the mixed meal. Twenty-four hours later, all patients ingested mixed meal followed by oral 8 g L-Arginine, and insulin levels were again measured at 0, 1, 2, 3, 4 and 5 h after the ingestion of the meal.
Insulin levels reached to peak values at the 2 (nd) hour, and decreased to baseline levels at the 5 (th) hour measurements both after the ingestion of mixed meal only and after the ingestion of mixed meal plus oral L-Arginine. First and 2 (nd) hour insulin levels were significantly higher after the ingestion of mixed meal plus oral L-Arginine.
In this study we used for the first time the combination of oral L-arginine with mixed meal test to evaluate the beta cell dysfunction in type 2 diabetic patients. Increments regarding serum insulin levels after the ingestion of mixed meal plus oral L-Arginine suggest that oral L-Arginine could be benefical for the evaluation of beta cell function and secretory defects.
明显的糖尿病发生需要胰岛素分泌缺陷。我们评估了新诊断的2型糖尿病患者摄入混合餐加口服L-精氨酸后胰腺β细胞的分泌反应。
本研究纳入了24例新诊断的2型糖尿病患者。所有患者均摄入了553千卡的混合餐。在混合餐前0小时以及混合餐摄入后1、2、3、4和5小时测量血清胰岛素水平。24小时后，所有患者先摄入混合餐，随后口服8克L-精氨酸，在餐食摄入后0、1、2、3、4和5小时再次测量胰岛素水平。
仅摄入混合餐以及摄入混合餐加口服L-精氨酸后，胰岛素水平均在第2小时达到峰值，并在第5小时测量时降至基线水平。摄入混合餐加口服L-精氨酸后，第1小时和第2小时的胰岛素水平显著更高。
在本研究中，我们首次使用口服L-精氨酸与混合餐试验相结合的方法来评估2型糖尿病患者的β细胞功能障碍。摄入混合餐加口服L-精氨酸后血清胰岛素水平的升高表明，口服L-精氨酸可能有助于评估β细胞功能和分泌缺陷。
