[N，N-二甲基甲酰胺暴露工人中谷胱甘肽S-转移酶T1、谷胱甘肽S-转移酶M1和细胞色素P450 2E1基因多态性与肝功能异常的关系]
[Abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide].
作者信息
Xu Cheng-Min, Qian Ya-Ling, Zhu Li-Jin, Xian Ju-Xiang, Chai Jian-Rong, Ruan Zheng, Zhang Xing
机构信息
Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Jun;27(6):333-7.
OBJECTIVE
To investigate abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide.
METHODS
Sixty-nine workers with abnormal liver function in a synthetic leather factory were recruited as case. One hundred and twenty five control subjects with similar work tasks were selected from the same factory. Genotypes for GSTT1 and GSTM1 were determined by multiplex PCR, and for CYP2E1 PstI by PCR-RFLP assay.
RESULTS
The frequency of positive GSTM1 was 59.42% in cases and 38.40% in control, with an odds ratio (OR) of 2.34,95% CI: 1.29-4.29 (P=0.005). For GSTT1 and CYP2E1 PstI, the frequencies of genotypes showed no significant difference between case and control.
CONCLUSION
GSTM1 positive genotype may be genetic risk factors for development of abnormal liver function in workers exposed to N, N-dimethylformamide.
目的
研究N，N - 二甲基甲酰胺暴露工人中谷胱甘肽S - 转移酶T1（GSTT1）、谷胱甘肽S - 转移酶M1（GSTM1）和细胞色素P450 2E1（CYP2E1）基因多态性与肝功能异常的相关性。
方法
选取某合成革厂69例肝功能异常工人作为病例组，从同一工厂选取125例工作任务相似的对照者。采用多重聚合酶链反应（PCR）检测GSTT1和GSTM1基因分型，采用聚合酶链反应 - 限制性片段长度多态性分析（PCR - RFLP）检测CYP2E1 PstI基因分型。
结果
病例组GSTM1阳性频率为59.42%，对照组为38.40%，优势比（OR）为2.34，95%可信区间（CI）：1.29 - 4.29（P = 0.005）。GSTT1和CYP2E1 PstI基因分型频率在病例组和对照组之间无显著差异。
结论
GSTM1阳性基因型可能是N，N - 二甲基甲酰胺暴露工人肝功能异常发生的遗传危险因素。
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