Krupadam Reddithota J, Bhagat Bhagyashree, Khan Muntazir S, Wate Prateek S
National Environmental Engineering Research Institute, Nagpur 440020, India.
J Nanosci Nanotechnol. 2009 Sep;9(9):5441-7. doi: 10.1166/jnn.2009.1142.
Molecularly imprinted polymers (MIPs) were synthesized using co-polymerization of methacrylic acid with cross-linking agent ethylene glycol dimethacrylate in the presence of benzo(alpha)pyrene (BAP) followed by the extraction of BAP from cross-linked polymer matrix. The MIP formed by this way contains nanopores, which are geometrically specific to the BAP molecule. BAP belongs to one of the carcinogenic airborne atmospheric pollutants. Morphological characteristics of MIP showed presence of nanopores with an average pore diameter of 27.19 A and Brunauer-Emmett-Teller (BET) surface area of 827.87 +/- 1.63 m2g(-1). The adsorption capacity of BAP onto MIP was determined using equilibrium experiments. The cross-reactivity (CR) experiments were also conducted to found selectivity of BAP in the presence of 5 other polyaromatic hydrocarbon (PAH) compounds. The selectivity factors were calculated for MIP, based on experimental data derived from CR studies. It was found that the PAH molecules smaller being in molecular length (pyrene) than BAP, showed higher interference (up to 40%) compared with other PAH compounds. By increasing the concentration of other PAH compounds (100 microg mL(-1)), the selectivity (alpha) of MIP was reduced from 3.5 to 1.6. The value of equilibrium binding constant for BAP, K(E) = 0.236 microg mL(-1), was larger than other PAH compounds (K(E) values range from 0.011 to 0.028 microg mL(-1)). These properties demonstrate that MIP prepared shows greater binding capacity and selectivity due to creation of homogenous nanopores patterned uniformly in the polymer matrix. This method of preparation of ordered materials at micro and nano-scale are useful in the field of nanodevices and sensors for environmental, defense and biomedical applications.
分子印迹聚合物（MIPs）通过在苯并（α）芘（BAP）存在下，使甲基丙烯酸与交联剂乙二醇二甲基丙烯酸酯进行共聚反应来合成，随后从交联聚合物基质中萃取BAP。通过这种方式形成的MIP包含纳米孔，这些纳米孔在几何形状上对BAP分子具有特异性。BAP属于致癌性空气传播大气污染物之一。MIP的形态特征显示存在平均孔径为27.19 Å且Brunauer-Emmett-Teller（BET）表面积为827.87±1.63 m²g⁻¹的纳米孔。使用平衡实验测定了BAP在MIP上的吸附容量。还进行了交叉反应性（CR）实验，以研究在存在其他5种多环芳烃（PAH）化合物的情况下BAP的选择性。基于CR研究得出的实验数据计算了MIP的选择性因子。结果发现，分子长度比BAP小的PAH分子（芘）与其他PAH化合物相比，表现出更高的干扰（高达40%）。通过增加其他PAH化合物的浓度（100 μg mL⁻¹），MIP的选择性（α）从3.5降至1.6。BAP的平衡结合常数K（E）= 0.236 μg mL⁻¹，大于其他PAH化合物（K（E）值范围为0.011至0.028 μg mL⁻¹）。这些特性表明，所制备的MIP由于在聚合物基质中均匀形成了均匀的纳米孔图案，从而显示出更大的结合能力和选择性。这种在微米和纳米尺度上制备有序材料的方法在用于环境、国防和生物医学应用的纳米器件和传感器领域中很有用。
