Petrov A I, Sukhorukov B I
Biofizika. 1977 Sep-Oct;22(5):924-6.
Possibility of spin label studies as a method of investigation of different molecular associations is considered with adenosine, adenosine-5'-mono, di- and triphosphate as examples. The proposed method is based on dependence of the parameters of spin-labelled derivatives ESR-spectra on concentration of corresponding unlabelled compounds. Being highly sensitive the method takes the advantage of obtaining not only thermodynamic characteristics of association but dynamic parameters of molecular mobility of the associates as well. It is shown by this method that base-phosphate interaction participates in nucleotide association, together with the stacking interaction.
以腺苷、腺苷-5'-单磷酸、二磷酸和三磷酸为例，探讨了自旋标记研究作为一种研究不同分子缔合方法的可能性。所提出的方法基于自旋标记衍生物ESR光谱参数对相应未标记化合物浓度的依赖性。该方法灵敏度高，不仅可以获得缔合的热力学特征，还可以获得缔合体分子迁移率的动力学参数。通过该方法表明，碱基-磷酸相互作用与堆积相互作用一起参与了核苷酸缔合。
