Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, 1-3, 119992 Moscow, Russian Federation.
Langmuir. 2009 Dec 1;25(23):13528-33. doi: 10.1021/la902031e.
Complexation of phospholipid lipsomes with a cationic polymer, poly(N-ethyl-4-vinylpyridinium bromide) (PEVP), and subsequent interliposomal migration of the adsorbed macromolecules, have been investigated. Liposomes of two different charge types were examined: (a) a liposomal system, with an overall charge near zero, consisting of zwitterionic phosphatidylcholine (egg lecithin, EL) with added doubly anionic phospholipid, cardiolipin (CL(2-)), and cationic dihexadecyldimethylammonium bromide (HMAB(+)), in a CL(2-)/HMAB(+) charge-to-charge ratio of 1:1; (b) an anionic liposomal system composed of an EL/CL(2-) mixture plus polyoxyethylene monocetyl ether (Brij 58). Both three-component systems were designed specifically to preclude liposomal aggregation upon electrostatic association with the PEVP, a phenomenon that had complicated analysis of data from several two-component liposomes. PEVP macromolecules were found from fluorescence experiments to migrate among the charge-neutral EL/CL(2-)/HMAB(+) liposomes. In the case of anionic EL/CL(2-)/Brij liposomes, a combination of fluorescence and laser microelectrophoresis methods showed that PEVP macromolecules travel from liposome to liposome while being electrostatically associated with anionic lipids.
已经研究了磷脂脂质体与阳离子聚合物聚（N-乙基-4-乙烯基吡啶溴化物）（PEVP）的络合，以及随后吸附大分子的脂质体间迁移。研究了两种不同电荷类型的脂质体：（a）一种带负电的脂质体系统，由两性离子磷脂酰胆碱（卵磷酯，EL）与外加的双阴离子磷脂心磷脂（CL（2-））和阳离子二十六烷基二甲基溴化铵（HMAB（+））组成，CL（2-）/HMAB（+）的电荷比为 1:1；（b）由 EL/CL（2-）混合物加聚氧乙烯单十六醚（Brij 58）组成的阴离子脂质体系统。这两种三组分系统都是专门设计的，以避免与 PEVP 静电结合时脂质体聚集，这种现象使对几个两成分脂质体的数据的分析变得复杂。荧光实验发现，PEVP 大分子在带中性电荷的 EL/CL（2-）/HMAB（+）脂质体之间迁移。对于阴离子 EL/CL（2-）/Brij 脂质体，荧光和激光微电泳方法的结合表明，PEVP 大分子在与阴离子脂质静电结合的同时从一个脂质体迁移到另一个脂质体。
