Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan.
J Am Chem Soc. 2009 Dec 23;131(50):18038-9. doi: 10.1021/ja908148z.
The duck-billed platypus (Ornithorhynchus anatinus) is one of the few venomous Australian mammals. We previously found that its crude venom potently induces Ca(2+) influx in human neuroblastoma IMR-32 cells. Guided by this bioassay, we identified 11 novel peptides, including the heptapeptide H-His-Asp-His-Pro-Asn-Pro-Arg-OH (1). Compounds 1-4 and 5-11 coincided with the 6-9 N-terminal residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP) and the 132-150 part of OvCNP precursor peptide, respectively. Heptapeptide 1, which is one of the primary components of the venom fluid (approximately 200 ng/microL), induced a significant increase in Ca(2+) in IMR-32 cells at 75 microM. To the best of our knowledge, this is the first example of the isolation of the N-terminal linear fragments of CNPs in any mammal.
鸭嘴兽（Ornithorhynchus anatinus）是少数几种有毒的澳大利亚哺乳动物之一。我们之前发现，其粗毒液能强烈诱导人神经母细胞瘤 IMR-32 细胞内钙离子流入。根据该生物测定，我们鉴定出 11 种新肽，包括七肽 H-His-Asp-His-Pro-Asn-Pro-Arg-OH（1）。化合物 1-4 和 5-11 分别与鸭嘴兽毒液 C 型利钠肽（OvCNP）的 6-9 N 端残基和 OvCNP 前体肽的 132-150 部分一致。七肽 1 是毒液液的主要成分之一（约 200ng/μL），在 75μM 时能显著增加 IMR-32 细胞内的[Ca（2+）]（i）。据我们所知，这是在任何哺乳动物中分离 CNP 的 N 端线性片段的首例。
