Aqua Bounty Technologies, 8395 Camino Santa Fe, Suite E, San Diego, California 92121, USA.
J Agric Food Chem. 2010 Feb 10;58(3):1429-33. doi: 10.1021/jf903349y.
Recombinant bovine somatotropin (rbST), also known as growth hormone, is used to enhance production and development of animals within the agriculture and aquaculture industries. Its use is controversial because of its potential effects on human and animal health. To screen for rbST in shrimp feed, a competitive enzyme-linked immunosorbent assay (ELISA) with an inhibition step was developed. Sample and rbST antibody (rabbit anti-rbST) were incubated at room temperature for 30 min. Subsequently, this competitive reaction was transferred to a microplate coated with rbST, using goat antirabbit IgG linked with horseradish peroxidise as the secondary antibody. Substrates for peroxidise were added, and the absorbance at 410 nm was determined. The applicability of the method was assessed using rbST extracted from "spiked" shrimp feed samples. The assay was reproducible and linear with R(2) values greater than 0.98 over the standard curve range of 20-500 microg/g. The intra- and interday precisions expressed as relative standard deviations were 3.4 and 5.3%, respectively. The mean recovery from 15 spiked feed samples was 105%. This assay will be a valuable tool for quantitative detection of rbST by both governments and commercial companies and can be modified for other types of feed.
重组牛生长激素（rbST），也称为生长激素，用于提高农业和水产养殖业动物的产量和发育。由于其对人类和动物健康的潜在影响，其使用存在争议。为了在虾饲料中筛选 rbST，开发了一种具有抑制步骤的竞争酶联免疫吸附测定（ELISA）。将样品和 rbST 抗体（兔抗 rbST）在室温下孵育 30 分钟。随后，将这种竞争反应转移到包被 rbST 的微孔板上，使用辣根过氧化物酶偶联的山羊抗兔 IgG 作为第二抗体。加入过氧化物酶的底物，并测定 410nm 处的吸光度。使用“添加”虾饲料样品中提取的 rbST 评估该方法的适用性。该测定具有重现性和线性，标准曲线范围为 20-500μg/g 时 R（2）值大于 0.98。日内和日间精密度分别表示为相对标准偏差分别为 3.4%和 5.3%。从 15 个添加饲料样品中的平均回收率为 105%。该测定将成为政府和商业公司定量检测 rbST 的有用工具，并且可以针对其他类型的饲料进行修改。
