Department of Laboratory Hematology, College of Laboratory Medicine of Dalian Medical University, Dalian, China.
Cancer Sci. 2010 Feb;101(2):379-86. doi: 10.1111/j.1349-7006.2009.01417.x. Epub 2009 Oct 27.
B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) is highly expressed in several malignant tumors and its expression level is positively correlated with tumor invasion, distant metastasis, prognosis, and recurrence. In the present study, the biological effect of small interfering RNA (siRNA) that targeted Bmi-1 expression was studied in human cervical carcinoma cell line HeLa cells. Bmi-1 siRNA inhibited the expression of Bmi-1 at the mRNA and protein levels in HeLa cells, which significantly affected proliferation, colony formation, and migration of HeLa cells in vitro and in vivo. Therefore, silencing Bmi-1 may be a potential therapeutic option for the management of some human cancers.
B 细胞特异性 Moloney 鼠白血病病毒插入位点 1(Bmi-1)在多种恶性肿瘤中高表达,其表达水平与肿瘤侵袭、远处转移、预后和复发呈正相关。本研究探讨了针对 Bmi-1 表达的小干扰 RNA(siRNA)在人宫颈癌 HeLa 细胞系中的生物学效应。Bmi-1 siRNA 抑制了 HeLa 细胞中 Bmi-1 的 mRNA 和蛋白表达,显著影响了 HeLa 细胞的体外和体内增殖、集落形成和迁移。因此,沉默 Bmi-1 可能是某些人类癌症治疗的潜在选择。