Yu Qi, Su Benli, Liu Dandan, Liu Ben, Fan Ying, Wang Yingyan, Meng Xiuxiang
Department of Laboratory Hematology, School of Laboratory Medicine, Dalian Medical University, Dalian 116027, P.R. China.
Oligonucleotides. 2007 Fall;17(3):327-35. doi: 10.1089/oli.2007.0070.
The oncogene Bmi-1 regulates cell proliferation and senescence. It is reported that it controlled the self-renewal of leukemic and breast cancer stem cell and was overexpressed in some solid tumors and hematologic malignancies. In this study, the effects of inactivation of Bmi-1 mediated by a plasmid-expressing antisense Bmi-1 RNA on the proliferation of lung cancer cell line A549 were investigated. As a result, when the plasmid was stably introduced into the cell line, the Bmi-1 protein level was specifically downregulated, and the cell proliferation was significantly inhibited as shown by the cell growth curve and colony forming assay. The cells were found mostly in the phase of G(0)/G(1) and cells in S phase were significantly decreased. Our results suggest that targeting Bmi-1 might be a therapeutic potential for the treatment of non-small-cell lung cancer.
致癌基因Bmi-1调节细胞增殖和衰老。据报道，它控制白血病和乳腺癌干细胞的自我更新，并且在一些实体瘤和血液系统恶性肿瘤中过表达。在本研究中，研究了表达反义Bmi-1 RNA的质粒介导的Bmi-1失活对肺癌细胞系A549增殖的影响。结果，当将该质粒稳定导入细胞系时，Bmi-1蛋白水平特异性下调，并且细胞生长曲线和集落形成试验表明细胞增殖受到显著抑制。发现细胞大多处于G(0)/G(1)期，S期细胞显著减少。我们的结果表明，靶向Bmi-1可能具有治疗非小细胞肺癌的潜力。
