Physiology Weihenstephan, Technical University Munich, Germany.
J Dairy Res. 2010 Feb;77(1):85-9. doi: 10.1017/S0022029909990355. Epub 2009 Nov 23.
The adaptation of a colorimetric technique for the analysis of cholesterol in raw milk is presented. Performance quality was satisfying (mean intra-assay coefficient of variation (CV) 4.8, inter-assay CV 9.1%, linearity between 0 and 7 mm, recovery of spiked cholesterol into raw milk 98.1 and 106.3%). However, the milk fat extraction must be carried out within the 48 hours following milk sampling. When performing sampling, the significant variation of milk cholesterol composition during the milking process has to be taken into account.
本文介绍了一种比色法在生乳胆固醇分析中的应用。该方法的性能令人满意（平均批内变异系数（CV）为 4.8%，批间 CV 为 9.1%，线性范围为 0 至 7mm，生乳中添加胆固醇的回收率为 98.1%和 106.3%）。然而，必须在采样后 48 小时内进行乳脂肪提取。进行采样时，必须考虑挤奶过程中生乳胆固醇组成的显著变化。
