Zhang Bo, Liu Ming-yong, Zhao Jian-hua
Department of Orthopaedics, Daping Hospital/ Research Institute of Surgery, Third Military Medical University, Chongqing, China.
Chin J Traumatol. 2009 Dec;12(6):379-83.
To observe the osteoinductive activity of demineralized bone matrix (DBM) and deproteinized bone (DPB) made from human avascular necrotic femoral head.
The femoral head was cut into pieces with the size of 3 mm x 3 mm x 5 mm, which were made into DBM and DPB. These two kinds of biomaterials were cocultured with human bone mesenchymal stem cells (hBMSCs). Monolayer cells without biomaterials were cultured as control. Proliferative activity of hBMSCs was evaluated on days 1, 3, 5, 7 and 14. The concentration of alkaline phosphatase (ALP), osteocalcin (OC), and Ca(2+) were detected on days 1, 7, 14 and 21.
Cells cultured in DBM showed higher proliferative activity than did in DPB and monolayer cells (F =39.773, P <0.01). DBM and DPB also had osteoinductive activity. The concentrations of ALP (F=93.162, P <0.01), OC (F =236.852, P < 0.01), Ca(2+)(F =80.711, P <0.01) of DBM group were significantly higher than that of DPB and control groups.
In vitro, DBM and DPB made from avascular necrotic femoral head have osteoinductive activity when cocultured with hBMSCs, and the former is stronger than the latter.
观察人股骨头缺血性坏死脱矿骨基质（DBM）和脱蛋白骨（DPB）的骨诱导活性。
将股骨头切成3mm×3mm×5mm大小的碎块，制成DBM和DPB。将这两种生物材料与人骨髓间充质干细胞（hBMSCs）共培养。以无生物材料的单层细胞培养作为对照。在第1、3、5、7和14天评估hBMSCs的增殖活性。在第1、7、14和21天检测碱性磷酸酶（ALP）、骨钙素（OC）和Ca²⁺的浓度。
在DBM中培养的细胞比在DPB和单层细胞中培养的细胞具有更高的增殖活性（F = 39.773，P <0.01）。DBM和DPB也具有骨诱导活性。DBM组的ALP（F = 93.162，P <0.01）、OC（F = 236.852，P <0.01）、Ca²⁺（F = 80.711，P <0.01）浓度显著高于DPB组和对照组。
在体外，由股骨头缺血性坏死制成的DBM和DPB与hBMSCs共培养时具有骨诱导活性，且前者强于后者。
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