双氧水对棉花（棉属）纤维引发延迟突变体中纤维起始的影响。
Effect of H2O2 on fiber initiation using fiber retardation initiation mutants in cotton (Gossypium hirsutum).
机构信息
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Weigang No. 1, 210095 Nanjing, Jiangsu Province, China.
出版信息
J Plant Physiol. 2010 Mar 15;167(5):393-9. doi: 10.1016/j.jplph.2009.10.005. Epub 2009 Nov 22.
Single-celled fibers initiate at anthesis from cotton seed epidermal cells of normal developmental cotton cultivars; however, fiber initiation is retarded in some cotton fiber mutants. In this study, the relationship between genes associated with fiber initiation retardation and fiber initiation development was investigated using three cotton fiber developmental mutants: recessive naked seed n2; dominant naked seed N1; and Xinxiangxiaoji Linted-Fuzzless Mutant (XinFLM); with genetic standard line TM-1 (TM-1) as control. Retardation during fiber initiation development was observed in N1 and XinFLM by scanning electron microscope (SEM) analysis. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of genes related to the fiber initiation development showed that the expression of GhEXP1 and GhMYB25 was lower in N1 and XinFLM than in TM-1 and n2, however, the expression of GhTTG1 and GhTTG3 in XinFLM and n2 was higher than in TM-1 and N1. In vivo and in vitro treatments on ovules demonstrated that 30% hydrogen peroxide (H2O2) could prevent fiber initiation retardation in XinFLM, but no evident effect on N1. To further confirm the relationship between gene expression and the effects of H2O2 in XinFLM, qRT-PCR analysis of four differentially expressed genes was performed using -1d post-anthesis (DPA) ovules of XinFLM treated for 24 and 48h with 30% H2O2 and H2O, respectively, with 0 and 1 DPA untreated ovules from XinFLM and TM-1 as control. The results showed that the expression of GhMYB25 and GhEXP1 showed significant difference in XinFLM after -1 DPA ovule treated for 24h relative to the untreated or H2O-treated ovules, with the expression of GhMYB25 increased significantly and that of GhEXP1 decreased. This implied that H2O2 might be one of the upstream signal molecules affecting the expression of GhMYB25 and GhEXP1 genes. The fiber initiation retardation in XinFLM might be related to the production of reactive oxygen species (ROS).
单细胞纤维在棉花种子表皮细胞中从开花期开始形成；然而，在一些棉花纤维突变体中，纤维起始会延迟。在这项研究中，使用三个棉花纤维发育突变体：隐性裸籽 n2；显性裸籽 N1；和新乡夏吉稀毛突变体（XinFLM）；以遗传标准系 TM-1（TM-1）作为对照，研究了与纤维起始延迟相关的基因与纤维起始发育之间的关系。通过扫描电子显微镜（SEM）分析观察到 N1 和 XinFLM 中纤维起始发育的延迟。对与纤维起始发育相关基因的反转录-聚合酶链反应（RT-PCR）分析表明，在 N1 和 XinFLM 中，GhEXP1 和 GhMYB25 的表达低于 TM-1 和 n2，然而，在 XinFLM 和 n2 中，GhTTG1 和 GhTTG3 的表达高于 TM-1 和 N1。在体内和体外对胚珠的处理表明，30%的过氧化氢（H2O2）可以防止 XinFLM 中的纤维起始延迟，但对 N1 没有明显影响。为了进一步证实基因表达与 XinFLM 中 H2O2 作用之间的关系，使用 30%H2O2 和 H2O 分别处理 XinFLM 的-1d 开花后（DPA）胚珠 24 和 48h，对 4 个差异表达基因进行 qRT-PCR 分析，以 0 和 1 DPA 未处理的 XinFLM 和 TM-1 胚珠作为对照。结果表明，在 XinFLM 中，-1 DPA 胚珠处理 24h 后，GhMYB25 和 GhEXP1 的表达与未处理或 H2O 处理的胚珠相比有显著差异，GhMYB25 的表达显著增加，而 GhEXP1 的表达则减少。这表明 H2O2 可能是影响 GhMYB25 和 GhEXP1 基因表达的上游信号分子之一。XinFLM 中的纤维起始延迟可能与活性氧（ROS）的产生有关。
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