Donovan-Peluso M, Acuto S, O'Neill D, Hom A, Maggio A, Bank A
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Blood. 1991 Feb 15;77(4):855-60.
We have constructed fusion genes comprised of gamma and beta globin elements and globin sequences linked to neomycin resistance (neoR) genes to define the cis acting sequences responsible for developmental stage-specific expression and induction of fetal globin genes in embryonic-fetal erythroleukemia K562 cells. The results indicate that the gamma promoter is required for proper initiation of transcription. However, the accumulation of gamma globin transcripts in response to hemin induction requires the additional presence of either gamma intervening sequence 2 or the 3' enhancer element of the beta globin gene. Thus, the gamma promoter may provide the elements for developmental stage-specific gene expression during fetal life. By contrast, the beta 3' enhancer is erythroid-specific but not developmental stage- or gene-specific.
我们构建了由γ和β珠蛋白元件以及与新霉素抗性（neoR）基因相连的珠蛋白序列组成的融合基因，以确定负责胚胎-胎儿红白血病K562细胞中发育阶段特异性表达和胎儿珠蛋白基因诱导的顺式作用序列。结果表明，γ启动子是转录正确起始所必需的。然而，响应于血红素诱导的γ珠蛋白转录本的积累需要γ内含子序列2或β珠蛋白基因的3'增强子元件的额外存在。因此γ启动子可能为胎儿期发育阶段特异性基因表达提供元件。相比之下，β 3'增强子是红系特异性的，但不是发育阶段或基因特异性的。
