精细定位中国荷斯坦牛群体 6 号染色体上影响牛奶生产性状的数量性状基因座。

Fine mapping quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population.

机构信息

State Key Laboratory for Agrobiotechnology, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

出版信息

J Genet Genomics. 2009 Nov;36(11):653-60. doi: 10.1016/S1673-8527(08)60157-5.

DOI:10.1016/S1673-8527(08)60157-5

PMID:19932461

Abstract

To fine map the previously detected quantitative trait loci (QTLs) affecting milk production traits on bovine chromosome 6 (BTA6), 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS690 to BM4528 were selected and 918 daughters of 8 sires were genotyped. Two mapping approaches, haplotype sharing based LD mapping and single marker regression mapping, were used to analyze the data. Both approaches revealed a quantitative trait locus (QTL) with significant effects on milk yield, fat yield and protein yield located in the segment flanked by markers BMS483 and MNB209, which spans a genetic distance of 0.6 cM and a physical distance of 1.5 Mb. In addition, the single marker regression mapping also revealed a QTL affecting fat percentage and protein percentage at marker DIK2291. Our fine mapping work will facilitate the cloning of candidate genes underlying the QTLs for milk production traits.

摘要

为了精细定位影响牛 6 号染色体（BTA6）上乳产量性状的先前检测到的数量性状位点（QTL），选择了位于从 BMS690 到 BM4528 的间隔 14.3cM 内的 15 个微卫星标记，对 8 个公牛的 918 个女儿进行了基因型分析。使用基于单倍型共享连锁不平衡的映射和单标记回归映射两种映射方法来分析数据。这两种方法都揭示了一个位于标记 BMS483 和 MNB209 之间的片段上的 QTL，该 QTL 对乳产量、脂肪产量和蛋白质产量有显著影响，其遗传距离为 0.6cM，物理距离为 1.5Mb。此外，单标记回归映射还揭示了一个影响标记 DIK2291 处脂肪百分比和蛋白质百分比的 QTL。我们的精细定位工作将有助于克隆乳产量性状 QTL 下的候选基因。