Olsen H G, Gomez-Raya L, Våge D I, Olsaker I, Klungland H, Svendsen M, Adnøy T, Sabry A, Klemetsdal G, Schulman N, Krämer W, Thaller G, Rønningen K, Lien S
Department of Animal Science, Agricultural University of Norway, N-1432 Aas, Norway.
J Dairy Sci. 2002 Nov;85(11):3124-30. doi: 10.3168/jds.S0022-0302(02)74400-7.
An autosomal genome scan for quantitative trait loci (QTL) affecting milk production traits was carried out on the Norwegian Dairy Cattle population. Six half-sibling families with a total of 285 sons organized according to a granddaughter design were analyzed by a multiple marker regression method. Suggestive QTL for one or several of the five milk traits (milk yield, protein percentage, protein yield, fat percentage and fat yield) were detected on chromosomes 3, 5, 6, 11, 13, 18 and 20. Among these results, the findings on chromosomes 3, 6, and 20 are highly supported by literature. The most convincing result was found close to marker FBN9 on chromosome 6, where a QTL was detected with alleles that cause a marked reduction in both protein and fat percentages and an increase in milk yield. The results for fat and protein percentage were highly significant even after accounting for multiple testing across the genome. Using bootstrapping, a 95% confidence interval for the position of the QTL for the percentage traits on chromosome 6 was estimated to 16 cM.
对挪威奶牛群体进行了常染色体基因组扫描，以寻找影响产奶性状的数量性状基因座（QTL）。采用多标记回归方法分析了按照孙女设计组建的6个半同胞家系，共285头公牛。在第3、5、6、11、13、18和20号染色体上检测到了与5种产奶性状（产奶量、蛋白质百分比、蛋白质产量、脂肪百分比和脂肪产量）中的一种或几种相关的潜在QTL。在这些结果中，第3、6和20号染色体上的发现得到了文献的高度支持。最有说服力的结果出现在第6号染色体上靠近标记FBN9的位置，在该位置检测到一个QTL，其等位基因导致蛋白质和脂肪百分比显著降低，产奶量增加。即使在考虑了全基因组的多重检验后，脂肪和蛋白质百分比的结果仍然高度显著。通过自展法，估计第6号染色体上百分比性状QTL位置的95%置信区间为16厘摩。
