Murakami N, Mehta P, Elzinga M
Laboratory of Neurobiochemistry, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314-6399.
FEBS Lett. 1991 Jan 14;278(1):23-5. doi: 10.1016/0014-5793(91)80074-d.
Antisera were produced against two synthetic peptides having sequences specific for cellular myosin heavy chains from human macrophages (peptide IIA) and bovine brain (peptide IIB). Immunoblots of tissue extracts were made with these antibodies, and they showed that mammalian cells have at least three distinct cellular myosin heavy chain isoforms. Two of the isoforms (MIIB1 and MIIB2) were recognized by antibodies against the peptide IIB, and the other (MIIA) by the anti-peptide IIA antibodies. Polyclonal anti-platelet myosin antibodies recognized the MIIA isoform, but did not recognize MIIB1 or MIIB2. The isoforms were expressed in a tissue-specific pattern; the MIIB1 isoform was found only in brain.
制备了针对两种合成肽的抗血清，这两种肽具有来自人类巨噬细胞（肽IIA）和牛脑（肽IIB）的细胞肌球蛋白重链特异性序列。用这些抗体对组织提取物进行免疫印迹，结果表明哺乳动物细胞至少有三种不同的细胞肌球蛋白重链同工型。其中两种同工型（MIIB1和MIIB2）可被抗肽IIB抗体识别，另一种（MIIA）可被抗肽IIA抗体识别。多克隆抗血小板肌球蛋白抗体可识别MIIA同工型，但不能识别MIIB1或MIIB2。这些同工型以组织特异性模式表达；MIIB1同工型仅在脑中发现。
