Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
Genome. 2009 Nov;52(11):957-67. doi: 10.1139/g09-070.
Drosophila melanogaster was used to identify genes with a potential role in genetic regulation of purine biosynthesis. In this study we examine two dominant genetic modifiers of the essential gene Prat, which encodes amidophosphoribosyltransferase (EC 2.4.2.14). We found that Mod(Prat:bw)3-1 enhances Prat expression only in female heads, whereas Mod(Prat:bw)3-5 suppresses Prat in all stages and tissues examined for both sexes. For Mod-3-5, gene expression microarrays were used to identify other genes that are affected by the modifier. Three mapping approaches were used to localize these modifiers. Deficiency and meiotic mapping showed that the complex lethal complementation group previously associated with Mod-3-1 and Mod-3-5 is actually due to shared second-site lethal mutations. Using male recombination mapping, Mod-3-1 was localized to a 21 kilobase region containing nine genes, and Mod-3-5 was localized to a 53 kilobase region containing eight genes.
黑腹果蝇被用于鉴定嘌呤生物合成的遗传调控中具有潜在作用的基因。在这项研究中，我们研究了两个显性遗传修饰因子对必需基因 Prat 的影响，该基因编码氨甲酰磷酸核糖基转移酶（EC 2.4.2.14）。我们发现 Mod(Prat:bw)3-1 仅在雌性头部增强 Prat 的表达，而 Mod(Prat:bw)3-5 则抑制两性所有研究阶段和组织中的 Prat。对于 Mod-3-5，我们使用基因表达微阵列来鉴定受修饰因子影响的其他基因。我们使用了三种作图方法来定位这些修饰因子。缺失和减数分裂作图表明，先前与 Mod-3-1 和 Mod-3-5 相关的复杂致死互补群实际上是由于共享的第二位点致死突变所致。使用雄性重组作图，将 Mod-3-1 定位到包含九个基因的 21 千碱基区域，将 Mod-3-5 定位到包含八个基因的 53 千碱基区域。
