National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement (Wuhan Branch), Huazhong Agricultural University, Wuhan 430070, China.
Genome. 2009 Sep;52(9):755-60. doi: 10.1139/g09-049.
9012AB, a recessive genic male sterility (RGMS) line derived from spontaneous mutation in Brassica napus, has been playing an important role in rapeseed hybrid production in China. The male sterility of 9012AB is controlled by two recessive genes (ms3 and ms4) interacting with one recessive epistatic suppressor gene (esp). The objective of this study was to develop PCR-based markers tightly linked to the esp gene and construct a high-resolution map surrounding the esp gene. From the survey of 512 AFLP primer combinations, 3 tightly linked AFLP markers were obtained and successfully converted to codominant or dominant SCAR markers. Furthermore, a codominant SSR marker (Ra2G08) associated with the esp gene was identified through genetic map integration. For fine mapping of the esp gene, these PCR-based markers were analyzed in a large BC1 population of 2545 plants. The esp gene was then genetically restricted to a region of 1.03 cM, 0.35 cM from SSR marker Ra2G08 and 0.68 cM from SCAR marker WSC6. The SCAR marker WSC5 co-segregated with the target gene. These results lay a solid foundation for map-based cloning of esp and will facilitate the selection of RGMS lines and their temporary maintainers.
9012AB 是甘蓝型油菜自发突变产生的隐性核雄性不育系，在中国油菜杂种生产中发挥了重要作用。9012AB 的雄性不育性由两个隐性基因（ms3 和 ms4）与一个隐性上位抑制基因（esp）互作控制。本研究旨在开发与 esp 基因紧密连锁的 PCR 标记，并构建 esp 基因周围的高分辨率图谱。在对 512 对 AFLP 引物组合的调查中，获得了 3 个紧密连锁的 AFLP 标记，并成功转化为共显性或显性 SCAR 标记。此外，通过遗传图谱整合，鉴定到与 esp 基因紧密连锁的共显性 SSR 标记 Ra2G08。为了对 esp 基因进行精细定位，对 2545 株的大 BC1 群体进行了这些基于 PCR 的标记分析。esp 基因被遗传限制在一个 1.03cM 的区域内，离 SSR 标记 Ra2G08 0.35cM，离 SCAR 标记 WSC6 0.68cM。SCAR 标记 WSC5 与目标基因共分离。这些结果为 esp 的图谱克隆奠定了坚实的基础，将有助于 RGMS 系及其临时保持系的选择。
