牛群 B 型轮状病毒 VP1、VP2、VP4、VP6、NSP1 和 NSP2 基因的分子特征：一种新型 VP4 基因型的鉴定。
Molecular characterization of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of bovine group B rotaviruses: identification of a novel VP4 genotype.
机构信息
Department of Hygiene, Sapporo Medical University School of Medicine, S 1,W 17, Chuo-Ku, Sapporo, Hokkaido 060-8556, Japan.
出版信息
Arch Virol. 2010 Feb;155(2):159-67. doi: 10.1007/s00705-009-0555-x.
Studies on bovine group B rotaviruses (GBRs) are limited. To date, only the VP6 gene of a single bovine GBR strain and the VP7 and NSP5 genes of a few bovine GBR strains have been sequenced and analyzed. In the present study, using a single-primer amplification method, we have determined the full-length nucleotide sequences of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of three bovine GBR strains from eastern India. In all six of these genes, the bovine GBR strains shared high genetic relatedness among themselves but exhibited high genetic diversity with cognate genes of human, murine and ovine GBRs. Interestingly, as with group A rotaviruses, the bovine GBR VP1, VP2, VP6 and NSP2 genes appeared to be more conserved than the VP4 and NSP1 genes among strains of different species. The present study provides important insights into the genetic makeup and diversity of bovine GBRs, and also identifies a novel GBR VP4 genotype.
关于牛群 B 轮状病毒（GBR）的研究有限。迄今为止，仅对单个牛 GBR 株的 VP6 基因和少数牛 GBR 株的 VP7 和 NSP5 基因进行了测序和分析。在本研究中，我们使用单引物扩增方法，确定了来自印度东部的三个牛 GBR 株的 VP1、VP2、VP4、VP6、NSP1 和 NSP2 基因的全长核苷酸序列。在这六个基因中，牛 GBR 株彼此之间具有很高的遗传相关性，但与人类、鼠类和羊类 GBR 的同源基因相比，具有很高的遗传多样性。有趣的是，与 A 组轮状病毒一样，牛 GBR 的 VP1、VP2、VP6 和 NSP2 基因在不同物种的株之间似乎比 VP4 和 NSP1 基因更保守。本研究提供了对牛 GBR 的遗传组成和多样性的重要见解，并确定了一种新型 GBR VP4 基因型。
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