Ghosh S, Varghese V, Samajdar S, Bhattacharya S K, Kobayashi N, Naik T N
Division of Virology, National Institute of Cholera and Enteric Diseases, Beliaghata, India.
Arch Virol. 2006 Jul;151(7):1329-44. doi: 10.1007/s00705-005-0714-7. Epub 2006 Feb 26.
A porcine Group A rotavirus strain (RU172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of Kolkata City, India. The G12 genotype specificity of RU172 was revealed by PCR-based genotyping assays following addition of a G12 type-specific primer (designed in our laboratory to pick up G12 isolates from field samples) and was confirmed by sequence analysis of the VP7-encoding gene. The RU172 strain exhibited maximum VP7 identities of 93.6% to 94.5% with human G12 strains at the deduced amino acid level. In spite of its G12 genotype nature, RU172 appeared to be distinct from human G12 rotaviruses and, on phylogenetic analysis, formed a separate lineage with human G12 strains. Among the other gene segments analyzed, RU172 belonged to NSP4 genotype B, had a NSP5 and VP6 of porcine origin, and shared maximum VP4 identities with porcine P[7] rotaviruses (94.3%-95.4% at the deduced amino acid level). Therefore, to the best of our knowledge, this is the first report of detection of an animal rotavirus strain with G12 genotype specificity. Detection of strains like RU172 provides vital insights into the genomic diversity of Group A rotaviruses of man and animals.
在印度加尔各答市郊区的一个养猪场进行的轮状病毒感染监测研究中，检测到一株猪A组轮状病毒（RU172）并对其进行了分子特征分析。在添加G12型特异性引物（在我们实验室设计，用于从现场样本中筛选G12分离株）后，通过基于PCR的基因分型检测揭示了RU172的G12基因型特异性，并通过对编码VP7基因的序列分析得到证实。在推导的氨基酸水平上，RU172毒株与人类G12毒株的VP7最大同源性为93.6%至94.5%。尽管具有G12基因型性质，但RU172似乎与人类G12轮状病毒不同，在系统发育分析中，它与人类G12毒株形成了一个单独的谱系。在分析的其他基因片段中，RU172属于NSP4基因型B，具有猪源的NSP5和VP6，并且与猪P[7]轮状病毒的VP4最大同源性（在推导的氨基酸水平上为94.3%-95.4%）。因此，据我们所知，这是首次报道检测到具有G12基因型特异性的动物轮状病毒株。检测像RU172这样的毒株为人类和动物A组轮状病毒的基因组多样性提供了重要见解。
