National AIDS Research Center, Bhosari, Pune 411026, Maharashtra, India.
Arch Virol. 2010;155(1):89-95. doi: 10.1007/s00705-009-0553-z. Epub 2009 Nov 21.
Sequence analysis of segment 2 (seg-2) of three Indian bluetongue virus (BTV) isolates, Dehradun, Rahuri and Bangalore revealed 99% nucleotide identity amongst them and 96% with the reference BTV 23. Phylogenetic analysis grouped the isolates in 'nucleotype D'. The deduced amino acid (aa) sequence of the Bangalore isolate showed a high variability in a few places compared to other isolates. B-cell epitope analyses predicted an epitope that is present exclusively in the Bangalore isolate. Two-way cross serum neutralization confirmed that Bangalore isolate is antigenically different from the other two isolates. The results of this study suggest that these three isolates are VP2 variants of BTV 23. This signifies that non-cross-neutralizing variants of the same BTV serotype should be included in vaccine preparation.
对印度三种蓝舌病毒（BTV）分离株德哈拉敦、拉胡里和班加罗尔的第 2 段（seg-2）进行序列分析显示，它们之间的核苷酸同一性为 99%，与参考 BTV 23 的核苷酸同一性为 96%。系统进化分析将这些分离株归为“核型 D”。与其他分离株相比，班加罗尔分离株的推导氨基酸（aa）序列在几个位置表现出高度变异性。B 细胞表位分析预测了一个仅存在于班加罗尔分离株中的表位。双向交叉血清中和证实，班加罗尔分离株与其他两种分离株在抗原性上存在差异。本研究结果表明，这三种分离株是 BTV 23 的 VP2 变体。这表明应在疫苗制备中包含相同 BTV 血清型的非交叉中和变体。
