Reelin 是一种血小板蛋白，作为纤维蛋白原上血小板铺展的正调节剂发挥作用。
Reelin is a platelet protein and functions as a positive regulator of platelet spreading on fibrinogen.
机构信息
Graduate Institute of Biomedical Sciences, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC.
出版信息
Cell Mol Life Sci. 2010 Feb;67(4):641-53. doi: 10.1007/s00018-009-0201-5. Epub 2009 Nov 21.
Abstract
Abnormalities of platelet functions have been linked to reelin-impaired neuronal disorders. However, little attention has been given to understanding the interplay between reelin and platelet. In this study, reelin was found to present in the human platelets and megakaryocyte-like leukemic cells. Reelin-binding assays revealed that extracellular reelin can interact with platelets through the receptor belonging to the low density lipoprotein receptor gene family. The reelin-to-platelet interactions enhance platelet spreading on fibrinogen concomitant with the augmentation of lamellipodia formation and F-actin bundling. In contrast, reelin has no effect on integrin alphaIIbbeta3 activation and agonist-induced platelet aggregation. Molecular analysis revealed that the up-regulation of Rac1 activity and the inhibition of protein kinase C delta-Thr505 phosphorylation are important for reelin-mediated enhancement of platelet spreading on fibrinogen. These findings demonstrate for the first time that reelin is present in platelets and the reelin-to-platelet interactions play a novel role in platelet signaling and functions.
摘要
血小板功能异常与 reelin 功能障碍性神经疾病有关。然而，人们对 reelin 和血小板之间的相互作用知之甚少。在这项研究中，发现 reelin 存在于人类血小板和巨核细胞样白血病细胞中。Reelin 结合分析表明，细胞外 reelin 可以通过属于低密度脂蛋白受体基因家族的受体与血小板相互作用。Reelin 与血小板的相互作用增强了血小板在纤维蛋白原上的铺展，同时增强了片状伪足的形成和 F- 肌动蛋白的捆绑。相比之下，Reelin 对整合素 alphaIIbbeta3 的激活和激动剂诱导的血小板聚集没有影响。分子分析表明，Rac1 活性的上调和蛋白激酶 C delta-Thr505 磷酸化的抑制对 reelin 介导的纤维蛋白原上血小板铺展的增强是重要的。这些发现首次表明，Reelin 存在于血小板中，Reelin 与血小板的相互作用在血小板信号转导和功能中发挥新的作用。
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