胶原改性光聚合物的制备：一种新型可生物降解的细胞生长凝胶。
Preparation of collagen modified photopolymers: a new type of biodegradable gel for cell growth.
机构信息
Faculty of Engineering, Department of Polymer Engineering, Yalova University, 77100 Yalova, Turkey.
出版信息
J Mater Sci Mater Med. 2010 Feb;21(2):761-75. doi: 10.1007/s10856-009-3929-4.
In this study a new branched methacrylated poly(propylene glycol-co-lactic acid) (PPG-PLA-IEM) and methacrylated cellulose acetate butyrate resin (CAB-IEM) were synthesized. Hydrogels with various amounts of PPG-PLA-IEM and CAB-IEM (25, 50 and 75 wt% IEM modified) were prepared by photopolymerization. Collagen tethered PEG-monoacrylate (PEGMA-collagen) was prepared and introduced as a bioactive moiety to modify the hydrogel in order to enhance cell affinity. In vitro attachment and growth of 3T3 mouse fibroblasts and human umbilical vein endothelial cells (HUVEC) on the hydrogels with and without collagen were also investigated. It was observed that, the collagen improves the cell adhesion onto the hydrogel surface. With the increasing amount of collagen, cell viability increased by 28% for ECV304 (P < 0.05) and 30% for 3T3 (P < 0.05).
在这项研究中，合成了一种新的支化甲基丙烯酰化聚（丙二醇-co-乳酸）（PPG-PLA-IEM）和甲基丙烯酰化醋酸丁酸纤维素树脂（CAB-IEM）。通过光聚合制备了具有不同量的 PPG-PLA-IEM 和 CAB-IEM（25、50 和 75wt%IEM 改性）的水凝胶。制备了胶原连接的聚乙二醇单丙烯酸酯（PEGMA-胶原），并将其作为生物活性部分引入到水凝胶中，以增强细胞亲和力。还研究了胶原存在和不存在的情况下，3T3 小鼠成纤维细胞和人脐静脉内皮细胞（HUVEC）在水凝胶上的体外附着和生长情况。结果表明，胶原改善了细胞在水凝胶表面的黏附。随着胶原含量的增加，ECV304 的细胞活力增加了 28%（P<0.05），3T3 的细胞活力增加了 30%（P<0.05）。
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