Akdemir Z Seden, Akçakaya Handan, Kahraman M Vezir, Ceyhan Taşkin, Kayaman-Apohan Nilhan, Güngör Atilla
Department of Chemistry, Marmara University, 34722 Goztepe-Istanbul, Turkey.
Macromol Biosci. 2008 Sep 9;8(9):852-62. doi: 10.1002/mabi.200700319.
In this study, photopolymerized hydrogels of fumarated poly(ethylene glycol) diglycidyl-co- poly(ethylene glycol) diacrylate have been synthesized and modified with cell adhesion peptide, Arg-Gly-Asp (RGD). The structural and mechanical properties of the hydrogels are found to be poly(ethylene glycol) diacrylate (PEGDA) dependent. The percentage of gelation is increased from 72 to 89 wt.-% when the amount of the crosslinker co-monomer (PEGDA) in the hydrogel formulation is increased from 20 to 40 wt.-%. In the present case, the equilibrium mass swelling is decreased from 216 to 93%. The viscosities of the uncured formulations have also been measured and likewise, the results were influenced by the increasing amount of PEGDA that reduced the value from 83 to 36 cP. The compressive modulus of the prepared hydrogels was improved with the addition of the PEGDA. Cell growth experiments have been performed by comparing the properties of the hydrogels with and without RGD units. The results show that RGD units enhance the adhesion of cells to the surface of the hydrogels. SEM-EDS studies reveal that nitrogen and calcium are produced on the osteoblast-seeded surface of the scaffold within the culture time period. [Figure: see text].
在本研究中，合成了富马酸化聚乙二醇二缩水甘油醚 - 聚乙二醇二丙烯酸酯的光聚合水凝胶，并用细胞黏附肽精氨酸 - 甘氨酸 - 天冬氨酸（RGD）进行了修饰。发现水凝胶的结构和力学性能取决于聚乙二醇二丙烯酸酯（PEGDA）。当水凝胶配方中交联剂共聚单体（PEGDA）的量从20 wt.-%增加到40 wt.-%时，凝胶化百分比从72 wt.-%增加到89 wt.-%。在本案例中，平衡质量溶胀从216%降至93%。还测量了未固化配方的粘度，同样，结果受PEGDA量增加的影响，其值从83 cP降至36 cP。添加PEGDA后，制备的水凝胶的压缩模量得到改善。通过比较有无RGD单元的水凝胶的性能进行了细胞生长实验。结果表明，RGD单元增强了细胞对水凝胶表面的黏附。扫描电子显微镜 - 能谱分析（SEM - EDS）研究表明，在培养时间段内，支架的成骨细胞接种表面产生了氮和钙。[图：见正文]
