Engineering School of Lorena, University of São Paulo, P.O. Box 116-12602-810, Lorena, São Paulo, Brazil.
Appl Biochem Biotechnol. 2010 May;161(1-8):372-81. doi: 10.1007/s12010-009-8858-7.
The aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. The reactor operated continuously for 22 days at 50 degrees C, and during the first 6 days, no significant decrease on the initial lipase activity was observed. Monoglycerides concentration was in the range from 25 to 33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivation profile described by Arrhenius model estimated values of 50 days and 1.37 x 10(-2) h(-1) the half-life and deactivation coefficient, respectively.
本研究的目的是在填充床连续反应器中，用固定化假单胞菌脂肪酶催化生物柴油副产物巴巴苏油进行甘油解反应。最佳反应条件先前已通过响应面法在分批实验中确定，其为甘油与油的摩尔比和反应温度的函数。在 50°C 下连续运行 22 天，在前 6 天，初始脂肪酶活性没有明显下降。单甘油脂浓度在 25 至 33 重量%之间。随后，检测到活性逐渐下降，并通过阿伦尼乌斯模型描述了失活动力学，估计半衰期和失活系数分别为 50 天和 1.37 x 10(-2) h(-1)。
