Li LiHua, Qiu XuHua, Li XiangHua, Wang ShiPing, Lian XingMing
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.
Sci China C Life Sci. 2009 Nov;52(11):1055-64. doi: 10.1007/s11427-009-0137-x. Epub 2009 Nov 24.
Phosphorus (P) is one of the most essential macronutrients required for plant growth. Although it is abundant in soil, P is often the limiting nutrient for crop yield potential because of the low concentration of soluble P that plants can absorb directly. The gene expression profile was investigated in rice roots at 6, 24 and 72 h under low P stress and compared with a control (normal P) profile, using a DNA chip of 60000 oligos (70 mer) that represented all putative genes of the rice genome. A total of 795 differentially expressed genes were identified in response to phosphate (Pi) starvation in at least one of the treatments. Based on the analysis, we found that: (i) The genes coding for the Pi transporter, acid phosphatase and RNase were up-regulated in rice roots; (ii) the genes involved in glycolysis were first up-regulated and then down-regulated; (iii) several genes involved in N metabolism and lipid metabolism changed their expression patterns; (iv) some genes involved in cell senescence and DNA or protein degradation were up-regulated; and (v) some transmembrane transporter genes were up-regulated. The results may provide useful information in the molecular process associated with Pi deficiency and thus facilitate research in improving Pi utilization in crop species.
磷（P）是植物生长所需的最重要的大量营养素之一。尽管土壤中磷含量丰富，但由于植物可直接吸收的可溶性磷浓度较低，磷往往是作物产量潜力的限制营养素。利用代表水稻基因组所有推定基因的60000个寡核苷酸（70聚体）的DNA芯片，研究了低磷胁迫下6、24和72小时水稻根中的基因表达谱，并与对照（正常磷）谱进行比较。在至少一种处理中，共鉴定出795个响应磷酸盐（Pi）饥饿而差异表达的基因。基于该分析，我们发现：（i）编码Pi转运蛋白、酸性磷酸酶和核糖核酸酶的基因在水稻根中上调；（ii）参与糖酵解的基因先上调后下调；（iii）一些参与氮代谢和脂质代谢的基因改变了它们的表达模式；（iv）一些参与细胞衰老以及DNA或蛋白质降解的基因上调；以及（v）一些跨膜转运蛋白基因上调。这些结果可能为与磷缺乏相关的分子过程提供有用信息，从而促进作物物种中提高磷利用率的研究。
