Wang YouHua, Feng Ying, Xu NaiYin, Chen BingLin, Ma RongHui, Zhou ZhiGuo
Key Laboratory of Crop Growth Regulation of the Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Sci China C Life Sci. 2009 Nov;52(11):1065-72. doi: 10.1007/s11427-009-0147-8. Epub 2009 Nov 24.
To investigate the response of key enzymes to nitrogen (N) rates in cotton fiber and its relationship with fiber strength, experiments were conducted in 2005 and 2006 with cotton cultivars in Nanjing. Three N rates 0, 240 and 480 kgN/hm(2), signifying optimum and excessive nitrogen application levels were applied. The activities and the gene expressions of the key enzymes were affected by N, and the characteristics of cellulose accumulation and fiber strength changed as the N rate varied. Beta-1,3-glucanase activity in cotton fiber declined from 9 DPA till boll opening, and the beta-1, 3-glucanase coding gene expression also followed a unimodal curve in 12-24 DPA. In 240 kgN/hm(2) condition, the characteristics of enzyme activity and gene expression manner for sucrose synthase and beta-1,3-glucanase in developing cotton fiber were more favorable for forming a longer and more steady cellulose accumulation process, and for high strength fiber development.
为研究棉花纤维中关键酶对施氮量的响应及其与纤维强度的关系，于2005年和2006年在南京对棉花品种进行了试验。设置了0、240和480 kgN/hm²三个施氮量水平，分别代表最佳施氮量和过量施氮量。关键酶的活性和基因表达受施氮量影响，随着施氮量的变化，纤维素积累特征和纤维强度也发生改变。棉花纤维中的β-1,3-葡聚糖酶活性从开花后9天到棉铃开裂逐渐下降，β-1,3-葡聚糖酶编码基因的表达在开花后12 - 24天也呈单峰曲线。在施氮量为240 kgN/hm²的条件下，发育中棉花纤维中蔗糖合酶和β-1,3-葡聚糖酶的酶活性和基因表达方式特征更有利于形成更长且更稳定的纤维素积累过程，以及有利于高强度纤维的发育。
