Casale T B, Abbas M K
Department of Internal Medicine, Veterans Affairs Medical Center, Iowa City, Iowa.
J Allergy Clin Immunol. 1991 Feb;87(2):565-74. doi: 10.1016/0091-6749(91)90016-h.
Platelet-activating factor (PAF)-induced neutrophil migration across endothelial and epithelial barriers is an important event in inflammation. We compared the effects of PAF on human neutrophil migration through filters alone, and human umbilical vein endothelial and Madin-Darby canine kidney epithelial cells cultured on these filters. We found that the amount of PAF-induced neutrophil migration was barrier dependent. At low PAF concentrations, neutrophil migration through filters exceeded migration measured through either cellular barrier. As PAF concentrations increased to greater than or equal to 1 mumol/L, neutrophil migration through human umbilical vein endothelial cells often equaled or exceeded migration measured through filters alone. In contrast, significant neutrophil migration through Madin-Darby canine kidney cells was not observed until PAF concentrations exceeded 50 mumol/L. Thus, the intensity of PAF-induced neutrophil inflammatory processes is, in part, dependent on the barrier through which the neutrophils must migrate.
血小板活化因子（PAF）诱导的中性粒细胞穿过内皮和上皮屏障的迁移是炎症中的一个重要事件。我们比较了PAF对人中性粒细胞单独穿过滤膜以及对培养在这些滤膜上的人脐静脉内皮细胞和Madin-Darby犬肾上皮细胞的迁移的影响。我们发现PAF诱导的中性粒细胞迁移量取决于屏障。在低PAF浓度下，中性粒细胞穿过滤膜的迁移超过了通过任何一种细胞屏障测得的迁移。随着PAF浓度增加到大于或等于1 μmol/L，中性粒细胞通过人脐静脉内皮细胞的迁移常常等于或超过单独通过滤膜测得的迁移。相反，直到PAF浓度超过50 μmol/L才观察到中性粒细胞通过Madin-Darby犬肾细胞的显著迁移。因此，PAF诱导的中性粒细胞炎症过程的强度部分取决于中性粒细胞必须穿过的屏障。
