Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA.
Electrophoresis. 2009 Nov;30(22):3897-909. doi: 10.1002/elps.200900215.
Cyclofructans (CFs) are a class of cyclic oligosaccharides with a crown ether skeleton. No enantioseparations have previously been reported using this class of chiral oligosaccharides in chromatography or electrophoresis. CFs and their sulfated derivatives were examined as chiral selectors using CE. The native CFs showed no enantioselectivity toward any tested compounds, while the sulfated CFs showed exceptional selectivity toward many cationic analytes, including primary, secondary, and tertiary amines and amino acids. Enantiomeric resolution factors as high as 15.4 were achieved within short analysis times (generally below 10 min). The effect of buffer type, buffer concentration, buffer pH, chiral selector concentration and organic modifier concentration was examined and optimized.
环化果糖（CFs）是一类具有冠醚骨架的环状低聚糖。在此之前，尚未有报道称使用这类手性低聚糖在色谱或电泳中进行对映体分离。CFs 及其硫酸化衍生物被用作 CE 中的手性选择剂进行了研究。天然 CFs 对任何测试化合物均无对映选择性，而硫酸化 CFs 对许多阳离子分析物表现出特殊的选择性，包括伯、仲和叔胺以及氨基酸。在较短的分析时间内（通常在 10 分钟以下），实现了高达 15.4 的对映体分辨率因子。考察并优化了缓冲液类型、缓冲液浓度、缓冲液 pH 值、手性选择剂浓度和有机溶剂浓度的影响。
