Yan Riming, Zhang Zhibin, Zhu Du, Chu Ju
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Sep;25(9):1352-9.
Metabolic flux analysis is a very powerful tool to understand CO2 fixation and light energy utilization of microalgae during photoautotrophic cultivation. A comprehensive network structure for the autotrophic growth of Synechococcus sp. PCC7942 was proposed, and the carbon and energetic metabolism under different incident light intensity was investigated based on metabolic flux analysis in this paper. These results showed that CO2 fixation was the main energy and reducing potential trap which accounted for 85% and 70% of the total energy and reducing potential consumption respectively. We also found that the cell yield and the maximum cell yield based on ATP synthesis were maintained 2.80 g/mol and 2.97 g/mol respectively under the appointed incident intensity. But the cell yield on absorbed light energy their corresponding energy conversion efficiency were descended with the increasing of incident intensity.
代谢通量分析是了解微藻在光合自养培养过程中二氧化碳固定和光能利用的一种非常强大的工具。本文提出了聚球藻属PCC7942自养生长的综合网络结构,并基于代谢通量分析研究了不同入射光强下的碳代谢和能量代谢。这些结果表明,二氧化碳固定是主要的能量和还原电位阱,分别占总能量消耗和总还原电位消耗的85%和70%。我们还发现,在指定的入射光强下,细胞产量和基于ATP合成的最大细胞产量分别维持在2.80 g/mol和2.97 g/mol。但随着入射光强的增加,基于吸收光能的细胞产量及其相应的能量转换效率下降。