Yu Haiying, Zhou Yongyan, Cheng Xiumin
Drug Testing and Analysis Center, School of Pharmacy, Shandong University, Jinan, China.
Se Pu. 2009 Jul;27(4):447-52.
A sensitive and specific method with high performance liquid chromatographic fingerprints was developed for controlling the quality of donkey-hide glue and tortoise-shell glue according to the liposoluble constituents. The samples were prepared with the liquid-liquid-liquid three-phase static extraction. The liposoluble constituents were analysed by reversed-phase high performance liquid chromatography with a gradient program and detected at 205 nm. The chromatograms were evaluated by similarity and cluster analysis. The common peaks of two glues were marked respectively. The differences between two glues were revealed by similarity and cluster analysis. The method has good repeatability and stability, and can be served as the means to identify different kinds of animal glue.
建立了一种灵敏、特异的高效液相色谱指纹图谱法，用于根据脂溶性成分控制阿胶和龟甲胶的质量。采用液-液-液三相静态萃取法制备样品。脂溶性成分采用反相高效液相色谱梯度洗脱程序进行分析，检测波长为205 nm。通过相似度和聚类分析对色谱图进行评价。分别标记了两种胶的共有峰。通过相似度和聚类分析揭示了两种胶之间的差异。该方法具有良好的重复性和稳定性，可作为鉴别不同种类动物胶的手段。
