含有与花椰菜花叶病毒35S RNA启动子相连的雀麦花叶病毒cDNA的质粒的感染性。

Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35S RNA promoter.

作者信息

Mori M, Mise K, Kobayashi K, Okuno T, Furusawa I

机构信息

Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan.

出版信息

J Gen Virol. 1991 Feb;72 ( Pt 2):243-6. doi: 10.1099/0022-1317-72-2-243.

DOI:10.1099/0022-1317-72-2-243

PMID:1993867

Abstract

Full-length biologically active cDNAs of brome mosaic virus genomic RNAs 1, 2 and 3 were constructed by joining cDNA fragments. The cDNAs were constructed so that, at the 5' ends, unique SnaBI sites were present at the site of initiation of transcription. The cDNAs were inserted between a modified cauliflower mosaic virus (CaMV) 35S RNA promoter and terminator regions derived from CaMV DNA, and cloned into pUC18. When a mixture of the plasmid DNAs was inoculated onto Chenopodium hybridum leaves, local lesions appeared 5 to 6 days later. However, no symptoms appeared in similarly inoculated barley plants. Plasmid cDNAs with extra sequences at the 5' end were infectious but RNAs transcribed from cDNAs with similar sequences were not.

摘要

通过连接cDNA片段构建了雀麦花叶病毒基因组RNA 1、2和3的全长生物活性cDNA。构建cDNA时，使其在5′端转录起始位点处存在独特的SnaBI位点。将cDNA插入修饰的花椰菜花叶病毒（CaMV）35S RNA启动子和源自CaMV DNA的终止子区域之间，并克隆到pUC18中。当将质粒DNA混合物接种到杂种藜叶片上时，5至6天后出现局部病斑。然而，在同样接种的大麦植株上未出现症状。在5′端带有额外序列的质粒cDNA具有感染性，但从具有相似序列的cDNA转录的RNA不具有感染性。