通过直接摩擦接种番茄丛矮病毒基因载体的完整质粒DNA将外源基因快速导入植物。
Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector.
作者信息
Scholthof H B
机构信息
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843, USA.
出版信息
J Virol. 1999 Sep;73(9):7823-9. doi: 10.1128/JVI.73.9.7823-7829.1999.
Abstract
Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mosaic virus 35S promoter and the hepatitis delta virus antigenomic ribozyme with a downstream nopaline synthase gene polyadenylation signal, established infections upon rub-inoculation of plants with intact plasmids. Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detectable levels of localized foreign gene expression in inoculated leaves. This is the first demonstration of an infectious DNA from a member of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-inoculation of miniprep DNA onto a variety of plant species.
摘要
番茄丛矮病毒（TBSV）的互补DNA（cDNA）位于修饰的花椰菜花叶病毒35S启动子与丁型肝炎病毒反基因组核酶之间，并带有下游胭脂碱合酶基因的聚腺苷酸化信号，通过用完整质粒摩擦接种植物可建立感染。应用这种方法产生了一种基于TBSV DNA的基因载体，该载体在接种叶片中产生了易于检测到的局部外源基因表达水平。这是番茄病毒科成员的感染性DNA的首次证明，该DNA允许在将小量制备的DNA直接摩擦接种到多种植物物种上后，通过TBSV快速介导外源基因表达。
相似文献
1
Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector.通过直接摩擦接种番茄丛矮病毒基因载体的完整质粒DNA将外源基因快速导入植物。
J Virol. 1999 Sep;73(9):7823-9. doi: 10.1128/JVI.73.9.7823-7829.1999.
2
Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana.基于番茄丛矮病毒的载体在替代宿主烟草中融合和非融合表达异源蛋白的构建。
Appl Microbiol Biotechnol. 2020 Oct;104(19):8413-8425. doi: 10.1007/s00253-020-10837-z. Epub 2020 Aug 24.
3
Using vectors derived from tomato bushy stunt virus (TBSV) and TBSV defective interfering RNAs (DIs).使用源自番茄丛矮病毒（TBSV）和TBSV缺陷干扰RNA（DIs）的载体。
Curr Protoc Microbiol. 2007 Nov;Chapter 16:Unit 16I.4. doi: 10.1002/9780471729259.mc16i04s7.
4
Host impact on the stability of a plant virus gene vector as measured by a new fluorescent local lesion passaging assay.利用新的荧光局部病斑传代测定法评估宿主对植物病毒基因载体稳定性的影响。
J Virol Methods. 2012 Feb;179(2):289-94. doi: 10.1016/j.jviromet.2011.11.004. Epub 2011 Nov 19.
5
A novel co-delivery system consisting of a Tomato bushy stunt virus and a defective interfering RNA for studying gene silencing.一种由番茄丛矮病毒和缺陷干扰RNA组成的用于研究基因沉默的新型共递送系统。
J Virol Methods. 2003 Jul;111(1):37-42. doi: 10.1016/s0166-0934(03)00149-6.
6
Tombusvirus-based vector systems to permit over-expression of genes or that serve as sensors of antiviral RNA silencing in plants.基于 Tumbus 病毒的载体系统，以允许基因的过表达或作为植物中抗病毒 RNA 沉默的传感器。
Virology. 2014 Mar;452-453:159-65. doi: 10.1016/j.virol.2013.12.031. Epub 2014 Jan 31.
7
Characterization of a DCL2-Insensitive Isolate Infecting .鉴定一株侵染. 的不敏感 DCL2 分离物
Viruses. 2020 Oct 2;12(10):1121. doi: 10.3390/v12101121.
8
Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector.通过马铃薯X病毒载体表达单个基因鉴定番茄丛矮病毒宿主特异性症状决定因素。
Plant Cell. 1995 Aug;7(8):1157-72. doi: 10.1105/tpc.7.8.1157.
9
In planta expression of HIV-1 p24 protein using an RNA plant virus-based expression vector.使用基于RNA植物病毒的表达载体在植物中表达HIV-1 p24蛋白。
Mol Biotechnol. 2000 Feb;14(2):99-107. doi: 10.1385/mb:14:2:99.
10
Quantitative analysis of efficient endogenous gene silencing in Nicotiana benthamiana plants using tomato bushy stunt virus vectors that retain the capsid protein gene.利用保留衣壳蛋白基因的番茄丛矮病毒载体对本氏烟草植株中有效的内源性基因沉默进行定量分析。
Mol Plant Microbe Interact. 2007 Jun;20(6):609-18. doi: 10.1094/MPMI-20-6-0609.
引用本文的文献
1
Identification of a novel mild isolate of areca palm necrotic spindle-spot virus (ANSSVm) lacking two cysteine proteases (HC-Pro1 and HC-Pro2).鉴定出一种新型的槟榔坏死纺锤斑病毒温和分离株（ANSSVm），其缺少两种半胱氨酸蛋白酶（HC-Pro1和HC-Pro2）。
Front Microbiol. 2025 Jul 15;16:1553892. doi: 10.3389/fmicb.2025.1553892. eCollection 2025.
2
Optimization of a Protocol for Launching Grapevine Infection with the Biologically Active cDNA Clones of a Virus.利用病毒生物活性cDNA克隆引发葡萄感染的实验方案优化
Pathogens. 2023 Nov 3;12(11):1314. doi: 10.3390/pathogens12111314.
3
Delivery of progeny virus from the infectious clone of cucumber green mottle mosaic virus and quantification of the viral load in different host plants.从黄瓜绿斑驳花叶病毒感染性克隆中释放子代病毒并对不同寄主植物中的病毒载量进行定量分析。
3 Biotech. 2023 Jun;13(6):209. doi: 10.1007/s13205-023-03630-y. Epub 2023 May 23.
4
Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.谷子条纹病毒：玉米和其他单子叶植物基因功能分析的工具。
Mol Plant Pathol. 2023 Jul;24(7):811-822. doi: 10.1111/mpp.13330. Epub 2023 Apr 10.
5
Infectious Clones of Tomato Chlorosis Virus: Toward Increasing Efficiency by Introducing the Hepatitis Delta Virus Ribozyme.番茄褪绿病毒的感染性克隆：通过引入丁型肝炎病毒核酶提高效率
Front Microbiol. 2021 Jul 26;12:693457. doi: 10.3389/fmicb.2021.693457. eCollection 2021.
6
Discovery of a Novel Member of the Genus from Soybean ( L. Merr.).从大豆（L. Merr.）中发现该属的一个新成员。
Pathogens. 2021 Feb 18;10(2):223. doi: 10.3390/pathogens10020223.
7
Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China.胡椒褪绿斑驳病毒，一种侵染中国云南省辣椒的新的重组杆状病毒。
Virol J. 2021 Feb 23;18(1):42. doi: 10.1186/s12985-021-01511-5.
8
Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana.基于番茄丛矮病毒的载体在替代宿主烟草中融合和非融合表达异源蛋白的构建。
Appl Microbiol Biotechnol. 2020 Oct;104(19):8413-8425. doi: 10.1007/s00253-020-10837-z. Epub 2020 Aug 24.
9
RNA sequencing data of tobacco inoculated with .接种了……的烟草的RNA测序数据
Data Brief. 2020 Jan 3;29:105106. doi: 10.1016/j.dib.2019.105106. eCollection 2020 Apr.
10
Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.第二代番木瓜花叶病毒载体：在番茄中稳定性提高及多种报告基因
Plant Methods. 2019 May 28;15:58. doi: 10.1186/s13007-019-0446-4. eCollection 2019.
本文引用的文献
1
Multicomponent RNA plant virus infection derived from cloned viral cDNA.多组分 RNA 植物病毒感染源自克隆的病毒 cDNA。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7066-70. doi: 10.1073/pnas.81.22.7066.
2
Plant virus gene vectors for transient expression of foreign proteins in plants.用于在植物中瞬时表达外源蛋白的植物病毒基因载体。
Annu Rev Phytopathol. 1996;34:299-323. doi: 10.1146/annurev.phyto.34.1.299.
3
Presentation of heterologous peptides on plant viruses: genetics, structure, and function.植物病毒上异源肽的展示：遗传学、结构与功能。
Annu Rev Phytopathol. 1997;35:67-86. doi: 10.1146/annurev.phyto.35.1.67.
4
The tomato bushy stunt virus replicase proteins are coordinately expressed and membrane associated.番茄丛矮病毒复制酶蛋白是协同表达且与膜相关的。
Virology. 1995 Apr 1;208(1):365-9. doi: 10.1006/viro.1995.1162.
5
RNA-mediated trans-activation of transcription from a viral RNA.RNA介导的从病毒RNA转录的反式激活
Science. 1998 Aug 7;281(5378):829-32. doi: 10.1126/science.281.5378.829.
6
Cloning of tobacco genes that elicit the hypersensitive response.引发过敏反应的烟草基因的克隆
Plant Mol Biol. 1998 Mar;36(5):681-90. doi: 10.1023/a:1005949304445.
7
Nucleotide sequence and infectivity of a full-length cDNA clone of panicum mosaic virus.黍花叶病毒全长cDNA克隆的核苷酸序列与感染性
Virology. 1998 Feb 1;241(1):141-55. doi: 10.1006/viro.1997.8939.
8
The enigma of pX: A host-dependent cis-acting element with variable effects on tombusvirus RNA accumulation.pX之谜：一种宿主依赖的顺式作用元件，对番茄丛矮病毒RNA积累具有可变效应。
Virology. 1997 Oct 13;237(1):56-65. doi: 10.1006/viro.1997.8754.
9
A CaMV 35S promoter driven cDNA clone of tobacco mosaic virus can infect host plant tissue despite being uninfectious when manually inoculated onto leaves.一个由花椰菜花叶病毒35S启动子驱动的烟草花叶病毒cDNA克隆，尽管人工接种到叶片上时没有传染性，但仍能感染宿主植物组织。
Arch Virol. 1997;142(1):183-91. doi: 10.1007/s007050050069.
10
Plasmid vector for cloning infectious cDNAs from plant RNA viruses: high infectivity of cDNA clones of tomato aspermy cucumovirus.用于克隆植物RNA病毒感染性cDNA的质粒载体：番茄不孕黄瓜病毒cDNA克隆的高感染性
J Gen Virol. 1997 May;78 ( Pt 5):1181-5. doi: 10.1099/0022-1317-78-5-1181.
Zotero 插件