Shamoon B M, Kay A, Dupont de Dinechin S, Galibert F, Mandart E
UPR 41 CNRS Recombinaisons Génétiques, Centre Hayem, Hôpital Saint-Louis, Paris, France.
J Gen Virol. 1991 Feb;72 ( Pt 2):421-5. doi: 10.1099/0022-1317-72-2-421.
Polyclonal antibodies directed against the preS2 and S domains of the woodchuck hepatitis virus (WHV) envelope proteins were prepared using synthetic peptides and fusion polypeptides as immunogens. They were tested by immunoblotting and immunoprecipitation of infected woodchuck sera and lysates of a eukaryotic cell line expressing WHV envelope proteins. Only one anti-peptide serum directed against the preS2 domain was reactive with WHV envelope proteins, recognizing the preS2 and preS1 proteins by their preS2 epitopes. With recombinant fusion proteins we generated several anti-S sera, which recognized all envelope proteins, and anti-preS2 antisera, which recognized the preS proteins. Results obtained with our antisera showed that sera of infected woodchucks lack the low glycosylated form (GP33) of the preS2 protein, unlike human hepatitis B virus.
使用合成肽和融合多肽作为免疫原，制备了针对土拨鼠肝炎病毒（WHV）包膜蛋白前S2和S结构域的多克隆抗体。通过对感染土拨鼠血清和表达WHV包膜蛋白的真核细胞系裂解物进行免疫印迹和免疫沉淀试验对其进行检测。只有一种针对前S2结构域的抗肽血清与WHV包膜蛋白发生反应，通过其前S2表位识别前S2和前S1蛋白。利用重组融合蛋白，我们制备了几种识别所有包膜蛋白的抗S血清，以及识别前S蛋白的抗前S2抗血清。我们的抗血清检测结果表明，与人类乙型肝炎病毒不同，感染土拨鼠的血清中缺乏前S2蛋白的低糖基化形式（GP33）。
