Anal Chem. 2010 Jan 1;82(1):36-40. doi: 10.1021/ac902144w.
This letter demonstrates a microfluidic platform for enumerating CD4+ T-lymphocytes from whole blood using chemiluminescence as a detection method. We microfabricated traps in a chamber and coated them with anti-CD4 antibody to isolate CD4+ T-cells. Based on cell surface-bound CD3 antibodies conjugated with horseradish peroxidase, incubation with chemiluminescent substrate produced a current in the photodetector that was proportional to the number of captured CD4+ T-cells. Analyzing 3 microL of whole blood, the platform exhibited high cell-capture efficiency and produced cell counts with high correlation to results obtained from flow cytometry. Compared to other lab-on-a-chip methods for CD4 counting, this method uses an instrument that requires no external light source and no image processing to produce a digitally displayed result only seconds after running the test.
这封信展示了一种使用化学发光作为检测方法的微流控平台，用于从全血中计数 CD4+T 淋巴细胞。我们在腔室内微制造了陷阱，并在其上涂覆了抗 CD4 抗体以分离 CD4+T 细胞。基于与辣根过氧化物酶偶联的细胞表面结合的 CD3 抗体，与化学发光底物孵育会在光电探测器中产生与捕获的 CD4+T 细胞数量成正比的电流。分析 3μL 的全血时，该平台表现出高细胞捕获效率，并产生与流式细胞术获得的结果高度相关的细胞计数。与其他用于 CD4 计数的芯片实验室方法相比，该方法使用的仪器无需外部光源和图像处理，在运行测试仅几秒钟后即可显示数字结果。
