Curlej Jozef, Bulla Jozef, Chrenek Peter
Department of Genetics and Animal Reproduction, Animal Production Research Centre, Nitra, Luzianky, Slovak Republic.
Zygote. 2010 Aug;18(3):203-7. doi: 10.1017/S0967199409990207. Epub 2009 Nov 26.
The aim of our study was to analyse chromosomal aneuploidy occurrence in rabbit oocytes and embryos. Chromosomal analysis was done in rabbit oocytes and rabbit preimplantation embryos at 2-, 4- and 8-cell stages derived from in vivo fertilization. For mitotic cycle synchronization at the metaphase stage, 2-, 4- and 8-cell embryos were incubated in k-DMEM medium, supplemented with colcemid (1 microg/ml), for 7, 12 or 13h respectively. Success of metaphase synchronization was at values of 100, 86.1 and 92.2% for 2-, 4- and 8-cell embryos respectively. Recovery rate of analysable metaphase plates was reached at 58.8%, 83.9% and 59.8% for 2-, 4- and 8-cell embryos and 100% for oocytes. Significant difference (p < 0.01) in aneuploidy rate between oocytes (40.7%) and 2-cell embryos (62.5%) was found. These results demonstrate higher efficiency of synchronization of embryo cells at the metaphase stage, what may contribute to elevating the proportion of analysable nuclei.
我们研究的目的是分析兔卵母细胞和胚胎中染色体非整倍体的发生情况。对体内受精获得的兔卵母细胞以及2细胞、4细胞和8细胞阶段的兔植入前胚胎进行染色体分析。为使有丝分裂周期同步于中期，将2细胞、4细胞和8细胞胚胎分别在添加了秋水仙酰胺（1微克/毫升）的k-DMEM培养基中孵育7小时、12小时或13小时。2细胞、4细胞和8细胞胚胎中期同步化的成功率分别为100%、86.1%和92.2%。2细胞、4细胞和8细胞胚胎可分析中期板的回收率分别为58.8%、83.9%和59.8%，卵母细胞的回收率为100%。发现卵母细胞（40.7%）和2细胞胚胎（62.5%）的非整倍体率存在显著差异（p < 0.01）。这些结果表明胚胎细胞在中期阶段的同步化效率更高，这可能有助于提高可分析细胞核的比例。
