Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, P.O. Box 135, GR-54124 Thessaloniki, Greece.
J Inorg Biochem. 2010 Feb;104(2):161-70. doi: 10.1016/j.jinorgbio.2009.10.017. Epub 2009 Oct 23.
The mononuclear nickel(II) complexes with the first-generation quinolone antibacterial agent oxolinic acid in the presence or absence of nitrogen-donor heterocyclic ligands (2,2'-bipyridine, 1,10-phenanthroline or pyridine) have been synthesized and characterized. The experimental data suggest that oxolinic acid acts as deprotonated bidentate ligand coordinated to Ni(II) ion through the ketone and carboxylato oxygens. The crystal structure of (2,2'-bipyridine)bis(oxolinato) nickel(II), 2 has been determined by X-ray crystallography. The cyclic voltammograms of the complexes recorded in dmso solution and in 1/2 dmso/buffer (containing 150mM NaCl and 15mM trisodium citrate at pH 7.0) solution have shown that in the presence of calf-thymus DNA (CT DNA) they can bind to CT DNA by the intercalative binding mode. UV study of the interaction of the complexes with CT DNA has shown that the complexes bind to CT DNA and bis(aqua)bis(oxolinato) nickel(II) exhibits the highest binding constant to CT DNA. Competitive study with ethidium bromide (EB) has shown that the complexes can displace the DNA-bound EB indicating that they bind to DNA in strong competition with EB. The complexes exhibit good binding propensity to human or bovine serum albumin protein having relatively high binding constant values.
单核镍（II）配合物与第一代喹诺酮类抗菌药氧氟沙星在存在或不存在氮供杂环配体（2,2'-联吡啶、1,10-菲啰啉或吡啶）的情况下被合成并进行了表征。实验数据表明，氧氟沙星作为去质子的双齿配体通过酮基和羧基氧与 Ni(II)离子配位。（2,2'-联吡啶）双（氧氟沙星）镍（II），2 的晶体结构通过 X 射线晶体学确定。在 dmso 溶液和 1/2 dmso/缓冲液（在 pH 7.0 时含有 150mM NaCl 和 15mM 三磷酸钠）溶液中记录的配合物的循环伏安图表明，在小牛胸腺 DNA（CT DNA）存在下，它们可以通过嵌入结合模式与 CT DNA 结合。配合物与 CT DNA 的相互作用的 UV 研究表明，配合物与 CT DNA 结合，双（水）双（氧氟沙星）镍（II）与 CT DNA 具有最高的结合常数。与溴化乙锭（EB）的竞争研究表明，配合物可以取代 DNA 结合的 EB，表明它们与 DNA 结合具有很强的竞争性。这些配合物对人或牛血清白蛋白蛋白具有良好的结合倾向，具有相对较高的结合常数值。
