Szymezak J, Lavalard E, Leroy N, Gillery P
Laboratoire de biochimie et de recherche pédiatriques, CHU de Reims, France.
Ann Biol Clin (Paris). 2009 Nov-Dec;67(6):705-10. doi: 10.1684/abc.2009.0381.
We have evaluated the conservation prior to HbA(1c) determination of three whole blood samples stored at -80 degrees C, -20 degrees C, 4 degrees C and 20 degrees C, for a maximal duration of one year. HbA(1c) was measured by an ion-exchange high performance liquid chromatography (HPLC) method (Variant II). We have analyzed the HbA(1c) value and the quality of the chromatographic separation for each sample. Storage of whole blood samples at -80 degrees C is good for at least one year. Storage at 4 degrees C is correct for two weeks, without major sample degradation. A more important and earlier degradation occurs at -20 degrees C. The conservation at 20 degrees C (room temperature) is very short. In conclusion, the temperatures of 4 and -80 degrees C are of interest for whole blood storage before HbA(1c) measurement, respectively for short and long term conservations. The temperatures of 20 and -20 degrees C are not recommended.
我们评估了三个全血样本在-80℃、-20℃、4℃和20℃下储存长达一年时间后，在测定糖化血红蛋白（HbA1c）之前的保存情况。采用离子交换高效液相色谱（HPLC）法（Variant II型）测定HbA1c。我们分析了每个样本的HbA1c值和色谱分离质量。全血样本在-80℃下储存至少一年效果良好。在4℃下储存两周是合适的，样本没有明显降解。在-20℃下会发生更严重且更早的降解。在20℃（室温）下保存时间非常短。总之，4℃和-80℃分别适用于HbA1c测量前全血的短期和长期保存。不建议使用20℃和-20℃。
