Rolandsson Olov, Marklund Stefan L, Norberg Margareta, Agren Asa, Hägg Erik
Department of Public Health, Clinical Chemistry, Umeå University, S-901 85 Umeå, Sweden.
Metabolism. 2004 Nov;53(11):1496-9. doi: 10.1016/j.metabol.2004.04.015.
The ability of glycated hemoglobin A(1c) (HbA(1c)) to predict diabetes is unknown, but could be evaluated by analyses on samples stored in biobanks. The stability of HbA(1c) in long-term stored samples is, however, unknown. Moreover, the effect of hemolysis on HbA(1c) in the general population is not assessed. To explore these questions HbA(1c) was determined in 3 groups (n = 717) of samples with storage times at -80 degrees C differing between 10 years and 2 months. The results were compared with HbA(1c) analyzed in fresh blood samples (n = 174). The subjects were free from diabetes and aged 40 to 60 years. HbA(1c) was analyzed by cation exchange high-performance liquid chromatography (HPLC). The mean HbA(1c) results for the fresh and long-term stored samples were 4.25% +/- 0.39 and 4.19% +/- 0.43, respectively (P = not significant [NS]). The HbA(1c) levels in fresh and 2-month stored samples were essentially equal. There was no correlation between HbA(1c) in the fresh samples and the hemolysis parameters reticulocytes, haptoglobin, or bilirubin. HbA(1c) is apparently stable in samples long-term stored at -80 degrees C and is not commonly affected by hemolysis in the general population. HbA(1c) analyzed on biobank samples could be used to assess the predictive value for future diabetes and relationship to other morbidity and mortality.
糖化血红蛋白A1c（HbA1c）预测糖尿病的能力尚不清楚，但可通过对生物样本库中储存样本的分析来评估。然而，长期储存样本中HbA1c的稳定性尚不清楚。此外，一般人群中溶血对HbA1c的影响也未得到评估。为探究这些问题，我们对3组（n = 717）样本中的HbA1c进行了测定，这些样本在-80℃下的储存时间在10年至2个月之间。将结果与新鲜血液样本（n = 174）中分析的HbA1c进行比较。受试者无糖尿病，年龄在40至60岁之间。通过阳离子交换高效液相色谱法（HPLC）分析HbA1c。新鲜样本和长期储存样本的平均HbA1c结果分别为4.25%±0.39和4.19%±0.43（P = 无显著性差异[NS]）。新鲜样本和储存2个月样本中的HbA1c水平基本相等。新鲜样本中的HbA1c与溶血参数网织红细胞、触珠蛋白或胆红素之间无相关性。HbA1c在-80℃长期储存的样本中显然是稳定的，并且在一般人群中通常不受溶血影响。对生物样本库样本分析的HbA1c可用于评估未来糖尿病的预测价值以及与其他发病率和死亡率的关系。
