Sanderson C E, Belov K, Deakin J E
University of Sydney, NSW, Australia.
Cytogenet Genome Res. 2009;127(1):21-5. doi: 10.1159/000260372. Epub 2009 Nov 20.
The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative genomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.
帚尾袋貂（Macropus eugenii）是一种有袋类动物模型，其基因组最近已测序至2倍覆盖深度。尽管这对于比较基因组研究是一个很好的资源，但如果要充分利用该资源，基因定位信息至关重要。在本研究中，从帚尾袋貂细菌人工染色体（BAC）文库中分离出包含关键免疫基因的帚尾袋貂BAC。使用荧光原位杂交（FISH）将包含T细胞受体（TCR）和免疫球蛋白（Ig）基因的BAC物理定位到帚尾袋貂染色体上。这些免疫基因在帚尾袋貂基因组中的位置与从染色体涂染数据预测的位置一致，突出了有袋类动物中这些重要免疫基因基因组背景的保守性。在帚尾袋貂中分离和定位这些关键免疫基因将有助于组装最近测序的低覆盖度基因组，并将序列分配到染色体上。
